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Publications

PUBLICATIONS

  1. Sachana, M., Flaskos, J., Alexaki, E., Glynn, P., Hargreaves, A.J. (2001) The Toxicity of Chlorpyrifos towards Differentiating Mouse N2a Neuroblastoma Cells. Toxicol. Én Vitro 15, 369-372.
  2. Sachana, M., Flaskos, J., Nikolaidis, E., Hargreaves, A.J., Alexaki-Ôzivanidou, E. (2001) Inhibition of Rat Platelet 5-Hydroxytryptamine Uptake by Chlorpyrifos and Carbaryl. Pharmacol. Toxicol. 89, 195-200.
  3. Sachana, M., Flaskos, J., Alexaki, Å., Hargreaves, A.J. (2003) Inhibition of Neurite Outgrowth in N2A Cells by Leptophos and Carbaryl: Effects on Neurofilament Heavy Chain, GAP-43 and HSP-70. Toxicol. In Vitro 17, 115-120.
  4. Sachana, M., Flaskos, J., Glynn, P., Hargreaves, A.J. (2004) Neuropathy Target Esterase Activity and its Inhibition by Phenyl Saligenin Phosphate and Leptophos in Cultured Mouse N2a Neuroblastoma Cells. Review of Clinical Pharmacology and Pharmacokinetics. 18, 267-271.
  5. Sachana, M., Flaskos, J., Hargreaves, A.J. (2005) Effects of Chlorpyrifos and Chlorpyrifos-methyl on the Outgrowth of Axon-like Processes, Tubulin and GAP-43 in N2a Cells. Toxicol. Mech. Method 15, 405-410.
  6. Hargreaves, A.J. Fowler, M.J., Sachana, M., Flaskos, J., Bountouri, M., Coutts, I.C., Glynn, P., Harris, W., McLean, W.G. (2006) Inhibition of Neurite Outgrowth in Differentiating Mouse N2a Neuroblastoma Cells by Phenyl Saligenin Phosphate: Effects on MAP Kinase (ERK 1/2) Activation, Neurofilament Heavy Chain Phosphorylation and Neuropathy Target Esterase Activity. Biochem Pharmacol. 71, 1240-1247.
  7. Flaskos, J., Sachana, M., Pen, M., Harris, W.C., Hargreaves, A.J. (2006) Effects of Phenyl Saligenin Phosphate on Phosphorylation of Pig Brain Tubulin in Vitro. Environ. Toxicol. Pharmacol. 22, 70-74.
  8. Flaskos, J., Harris, W., Sachana, M., Munoz, D., Tack, J., Hargreaves, A.J. (2007) The Effects of Diazinon and Cypermethrin on the Differentiation of Neuronal and Glial Cell Lines. Toxicol. Appl. Pharmacol. 219, 172-180.
  9. Sachana, M., Flaskos, J., Sidiropoulou, E., Yavari, C.A., Hargreaves, A.J. (2008) Inhibition of Extension Outgrowth in Differentiating Rat C6 Glioma Cells by Chlorpyrifos and Chlorpyrifos Oxon: Effects on Microtubule Proteins. Toxicol. In Vitro 22, 1387-1391.
  10. Sidiropoulou, E., Sachana, M., Flaskos, J., Harris, W., Hargreaves, A.J., Woldehiwet, Z. (2009) Diazinon Oxon Affects the Differentiation of Mouse N2a Neuroblastoma Cells. Arch. Toxicol. 83, 373-380.
  11. Harris, W., Sachana, M., Flaskos, J., Hargreaves, A.J. (2009) Proteomic Analysis of Differentiating Neuroblastoma Cells Treated with Sub-lethal Neurite Inhibitory Concentrations of Diazinon: Indentification of Novel Biomarkers of Effect. Toxicol. Appl. Pharmacol. 240, 159-165.
  12. Harris, W., Sachana, M., Flaskos, J., Hargreaves, A.J. (2009) Neuroprotection from Diazinon-Induced Toxicity in Differentiating Murine N2a Neuroblastoma Cells. Neurotoxicology. 30, 958-964.
  13. Sidiropoulou, E., Sachana, M., Flaskos, J., Harris, W., Hargreaves, A.J., Woldehiwet, Z. (2009) Diazinon Oxon Interferes with Differentiation of Rat C6 Glioma Cells. Toxicol. In Vitro. 23, 1548-1552.
  14. Guitart, R., Croubels, S., Caloni, F., Sachana, M., Davanzo, F., Vandenbroucke, V., Berny, P. (2010) Animal poisoning in Europe. Part 1: Farm Livestock and Poultry. Vet. J. 183, 249-254.
  15. Berny, P., Caloni, F., Croubels, S., Sachana, M., Vandenbroucke, V., Davanzo, F., Guitart, R. (2010) Animal poisoning in Europe. Part 2: Companion Animals. Vet. J. 183, 255-259.
  16. Guitart, R., Sachana, M., Caloni, F., Croubels, S., Vandenbroucke, V., Berny, P. (2010) Animal poisoning in Europe. Part 3: Wildlife. Vet. J. 183, 260-265.

BOOK CHAPTERS

  1. Sachana, M., Hargreaves, A.J. (2007) Toxicological testing: in vivo and in vitro models. In: Gupta, R. C. (Ed), Veterinary Toxicology: Basic and Clinical Principles. Elsevier Inc., New York, pp. 51-66.
  2. Flaskos, J., Sachana, M., Developmental neurotoxicity of anticholinesterase pesticides. In press, Satoh, T., Gupta, R. C. (Eds), Anticholinesterase Pesticides: Metabolism, Neurotoxicity, and Epidemiology. John Wiley and Sons Inc., New York.
  3. Sachana, M., Flaskos, J., Hargreaves, A.J. In vitro biomarkers of developmental neurotoxicity. In press, Gupta, R. C. (Ed), Reproductive and Developmental Toxicology Elsevier Inc., New York.
  4. Hargreaves, A.J., Sachana, M., Flaskos, J. Cell differentiation and neurite outgrowth in continuous cell lines. In press, Aschner, M., Price A., Sunol C. (Eds), Neuromethods Springer Science Inc., New York.

SHORT COMMUNICATIONS

Sachana, M., Flaskos, J., Harris, W., and Hargreaves A.J. (2006). Neurotoxicological effects of diazinon, cypermethrin and their mixture in N2a neuroblastoma and glioma cell lines. J Vet Pharmacol Theurap 29: 199.

Sachana, M., Flaskos, J., and Hargreaves A.J. (2005) Effects of chlorpyrifos and chlorpyrifos methyl on cytoskeletal proteins and MAP kinase (ERK1/2) activation in N2a cells. Toxicology 213: 263.

Sachana, M., Harris, W., Tack, J., Flaskos, J., and Hargreaves A.J. (2005) Effects of diazinon and cypermethrin on differentiating neuronal and glial cell lines. Toxicol Sci 84: 399.

Sachana, M., Flaskos, J., and Hargreaves A.J. (2004) Effects of chlorpyrifos on neurofilament network and MAP kinase (ERK 1/2) activation in N2a cells. Toxicol Appl Pharmacol 197: 322.

Sachana, M., Flaskos, J., Boutouri, M., and Hargreaves A.J. (2003) Potential molecular markers related to the neurotoxicity of chlorpyrifos and chlorpyrifos-methyl in N2a neuroblastoma cells. J Vet Pharmacol Theurap 26: 136.

PRESENTATIONS AT SCIENTIFIC MEETINGS

1999
7th Meeting of the International Neurotoxicology Association, Leicester, UK.
Effects of chlorpyrifos and carbaryl on rat MAO-A and AChE
M Sachana, J Flaskos, E Nikolaidis, AJ Hargreaves, E Alexaki-Tzivanidou

1999
"Beyond the Genome" International Congress of Biochemistry and Molecular Biology, Birmingham, UK.
The effects of leptophos and carbaryl on differentiating N2a neuroblastoma cells.
M Sachana, J Flaskos, E Alexaki, AJ Hargreaves

2000
Invitox 2000. Workshop in Pueblo Acantilado, Spain.
The toxicity of chlorpyrifos towards differentiating mouse N2a neuroblastoma cells.
M Sachana, J Flaskos, E Alexaki, P Glynn, AJ Hargreaves

2003
9th EAVPT Congress. Lisbon, Portugal.
Potential molecular markers related to the neurotoxicity of chlorpyrifos and chlorpyrifos-methyl in N2a neuroblastoma cells (Oral presentation)
M Sachana, J Flaskos, AJ Hargreaves

2004
10th International Congress of Toxicology, Tampere, Finland.
Effects of chlorpyrifos on neurofilament network and MAP Kinase (ERK 1/2) activation in N2a cells.
M Sachana, J Flaskos, AJ Hargreaves

2004
Consortium for Post Genome Science, Manchester, UK.
Effects of phenyl saligenin phosphate on the phosphorylation of á-tubulin in vitro.
M Sachana, J Flaskos, Pen M, WC Harris, AJ Hargreaves

2004
Adverse reactions to drugs and chemicals: Studies from molecule to man, Joint BPS/BTS meeting: Liverpool, UK.
Effects of chlorpyrifos and chlorpyrifos-methyl on cytoskeletal proteins and MAP kinase (ERK 1/2) activation in N2a cells.
M Sachana, J Flaskos, AJ Hargreaves

2005
44th SOT meeting: New Orleans, USA.
Effects of diazinon and cypermethrin on differentiating neuronal and glial cell lines.
M Sachana, W Harris, J Tack, J Flaskos, AJ Hargreaves

2005
10th Meeting of the International Neurotoxicology Association. Finland.

a. The effects of phenyl saligenin phosphate in differentiating mouse N2a neuroblastoma cells.
M Sachana, AJ Hargeaves, MJ Fowler, M Boutouri, J Flaskos, W Harris, P Glynn, WG McLean

b. The effects of chlorpyrifos and fipronil on differentiating rat C6 glioma cells.
E Sidiropoulou, M Sachana, CA Yavari, J Flaksos, Z Woldehiwet, AJ Hargreaves

2005
30th FEBS Congress and 9th IUBMB Conference, Budapest, Hungary.
Effects of diazinon and cypermethrin on differentiating N2a and C6 cell lines.
AJ Hargreaves, W Harris, J Tack, M Sachana, J Flaskos

2006
31st FEBS Congress, Istanbul, Turkey.
Effects of diazinon on differentiating N2a cells: a proteomic approach.
AJ Hargreaves, W Harris, M Sachana, J Flaskos

2006
10th EAVPT Congress. Turin, Italy.
Neurotoxicological effects of diazinon, cypermethrin and their mixture in N2a neuroblastoma and C6 glioma cell lines
M Sachana, J Flaskos, W Harris, AJ Hargreaves

2006
Invitox 2006. Workshop in Ostend, Belgium
Chlorpyrifos and chlorpyrifos oxon exert direct toxic effects on differentiating rat C6 glioma cells
M Sachana, J Flaksos, E Sidiropoulou, CA Yavari, AJ Hargreaves

2006
International Conference on Food Contaminants and Neurodevelopmental disorders, Valencia, Spain
Proteomic analysis of the effects of diazinon and cypermethrin on differentiating mouse N2a cells
W Harris, M Sachana, J Flaskos, AJ Hargreaves

2007
11th Meeting of the International Neurotoxicology Association. California, USA.
Neuroprotection from diazinon-induced toxicity in differentiated murine N2a neuroblastoma cells (Oral presentation)
M Sachana, W Harris, J Flaskos, AJ Hargreaves

2007
25th SSCT Workshop in Salzau, Germany
Inhibition of the outgrowth of extensions in differentiating rat C6 glioma cells by chlorpyrifos and chlorpyrifos_oxon; effects on microtubule network (Oral presentation)
M Sachana, J Flaksos, E Sidiropoulou, CA Yavari, AJ Hargreaves

2008
15th International Congress on In Vitro Toxicology, Stockholm, Sweden
Diazinon Oxon Interferes with Differentiation of Rat C6 Glioma Cells.
Sidiropoulou, E., Sachana, M., Flaskos, J., Harris, W., Hargreaves, A.J., Woldehiwet, Z.

2008
EUROTOX, Rhodes, Greece.
Diazinon Oxon Affects the Differentiation of Mouse N2a Neuroblastoma Cells.
Sidiropoulou, E., Sachana, M., Flaskos, J., Harris, W., Hargreaves, A.J., Woldehiwet, Z.

2009
7thWorld Congress on Alternatives & Animal Use in the Life Sciences, Rome, Italy.
Veterinary Students' Perspectives on Alternative Methods Used in Teaching: Results from a Questionnaire in Two European Universities.
Caloni, F., Sachana, M.

2010
Inhibition of Neurite Outgrowth in Differentiating Mouse N2a Neuroblastoma Cells by Fipronil: Effects on MAP Kinase (ERK 1/2) Activation
Sidiropoulou, E., Sachana, M., Flaskos, J., Hargreaves, A.J., Woldehiwet, Z.

OTHER SCIENTIFIC MEETINGS ATTENDED

2002
Developmental Toxicity: using in vitro models to gain new mechanistic and predictive insights in Syngenta CTL, Alderley Park, Macclesfield, Cheshire, UK.

2005
Conference "Alternative approaches to animal testing", Brussels,Belgium.

RELEVANT COURSES ATTENDED

2003: "Omics in Toxicology"
2003: Applying for research funding
2004: Effective Interviewing Skills for Interviewers
2004: "Building better QSARs"
2004: Trends in quantitative RT- PCR and gene expression analysis
2004: Trends in differential expression proteomics
2004: New tools and techniques for protein research
2005: Alternative methods to animal toxicity testing