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Evangelos Barbas
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CURRICULUM VITAE

 

 

NAME:                          Evangelos BARBAS

CURRENT POSITION:         Assistant Professor

LABORATORY:                  Forest Genetics and Tree Improvement

DEPARTMENT:                 Forest  Production and Conservation

FACULTY:                       School of Forestry and Natural Environment

Aristotle  University of  Thessaloniki, Greece

 

Phone:                                                                   +302310992755

Fax:                                                                        +302310992777

Cell. Phone:                                                          +306972214785

e-mail:                                                  vbarbas@for.auth.gr

 

 

Education:

 

1992 University Pierre et Marie Curie (PARIS VI), PhD, Plant Biology and Physiology.

Walnut hybrids (Juglans nigra χ Juglans regia) development during tissue culture in relation to the transport of inorganic P.

Supervisors: Mme A. NOUGAREDE (PARIS VI), M. D. CORNU (INRA)

Jury: Mme NOUGAREDE A., Μ. BEN BADIS Α., Μ. FAVRE J.M. Μ.CHAILLOU S.,  Μ. CORNU D., Μ.LAMAZE Τ.

1987 University Pierre et Marie Curie (PARIS VI), MSc (D.E.A.), Plant Biology and Physiology. Option cytology and experimental morphogenesis.

“Contribution to the tissue culture of Douglas fir: Influence of different media on the growth.”

Jury : Mme NOUGAREDE Α., Μ. BEN BADIS A., Mme CZANINSKI Υ., Μ. LANDRE P.,  M. MONEGER R., M. PERBAL G., Mlle POUX N., M. ROLAND J.C, M. ROUSSAUX J.

Supervisor M. A. FRANCLET, Laboratoire de Biotechnologie des Essences   Forestières, AFOCEL,  Domaine de l'Etançon, Nangis.

1985: School of Forestry and Natural Environment. Bachelors degree Aristotle  University of  Thessaloniki, Greece

 

 

Post doctoral education:

 

2003-2004     INRA Unité Mixte de Recherche (INRA, INH, Université d’Angers) Génétique et Horticulture (GenHort),

     INRA, 49071    Beaucouzé, France (6 months)

     Gene cloning and sequencing. Elaboration of genetic maps, estimation of genetic variation with SSRs

1993-1994       Université d’Angers Laboratoire de Phytonique, 2 Bd. Lavoisier, 49045 Angers cedex, France. (12 months)

Introduction and continuous culture of mycorrhizal fungi in monoxenic conditions Plant- fungus Interactions             Early biochemical modifications during plant-fungus recognition.

Professional experience:

2007-2010:   Assistant Professor  Laboratory of Forest Genetics and Tree Improvement, School of Forestry and natural environment, Aristotle  University of  Thessaloniki,

1999-2007: Lecturer Laboratory of Forest Genetics and Tree Improvement, School of Forestry and natural environment, Aristotle  University of  Thessaloniki, Greece

1995-1998: Teaching and research, University of Angers, France

1993-1994    Post doc at the university of Angers, France

1988-1992    PhD, Forest Tree Breeding Center, Orléans, INRA

1986-1987    MSc, Laboratory of Biotechnology, Domaine de l’Etançon, AFOCEL

 

International Mobility:

Member of the Working Group II Maturation. Genosilva COST Ε-28

Deputy  national representative in the conifer network EUFORGEN

University Teaching, University of Angers (Erasmus project), France

Erasmus (EU-funded student mobility project), University Pierre et Marie Curie, France

Erasmus (EU-funded student mobility project), University of Angers, France

 

Research techniques:

Tissue culture                          Micropropagation, cell culture, somatic embryogenesis, protoplasts, artificial seeds

 

Molecular biology:                    Genetic mapping, genetic variation (SSRs)

 

Fungi culture:                          Monoxenic cultures of arbuscular mycorrhizal fungi, Pure ectomycorrhizal fungi cultures

 

Histology-Histochemistry:        Localisation of phenolics, hydrophobe tissue layers (callose, suberine), ectomycorrhizas,  arbuscular mycorrhizas

 

Biochemistry:                           Extraction, purification and separation of phenolic compounds (HPLC, TLC), mineral analysis of plant tissues (Torche à plasma, HPLC), 32P, 45Ca uptake and transport.

 

Grands:                   Hellenic Scholarship Foundation , ΙNRA, European Union

Selected publications:

 

Barbas Ε., Chaillou S., Cornu D., Doumas P., Jay-Allemand C. and Lamaze T. 1993 Orthophosphate nutrition of in vitro propagated hybrid walnut (Juglans nigra χ Juglans regia) trees: Pi (32Pi) uptake and transport in relation to callus and shoot development, Plant Physiol Biochem 31 (1) : 41-2.

 

Barbas E., Jay-Allemand C, Doumas P., Chaillou S. and Cornu D. 1993 Effects of gelling agents on growth, mineral composition and naphtoquinone content of in vitro explants of hybrid walnut trees (Juglans nigra χ Juglans regia), Ann Sci For  50 : 177-186

 

Strullu D.G., Declerck S., Plenchette C, Barbas E. and Moreau J.C. 1995 Un inoculum pur de mycorrhizes AM pour le bananier. Journée SFPV-PRIA-BHR, Angers 20-21 février 1996

 

Feugey L., Barbas Ε., Viemont J.D., Strullu D.G. and Simoneau  Ρ. 1995 Plant  defense induction during the mycorrhizal symbiosis. 4e Forum des Jeunes Chercheurs en Physiologie Végétale : Génome et Physiologie Intégrée des Plantes. SFPV Septembre 1995, Clermond Ferrand.

 

Barbas E., Tavenard R.και Strullu D.G. 2000 Somatic embryos artificial seeds including  Glomus intraradices mycorrhizal fungi spores. Proceedings of the 8th Congress of the Hellenic Scientific Society of Plant Breeding, 23-25 October, Arta, σ. 155-164.

 

Barbas E. και Strullu D. G. 2002 Isolation and in vitro culture of arbuscular fungi. Proceedings of the  9ου Congress of the Hellenic Scientific Botanical Society, 9-12 may 2002, Argostoli, p. 206-211.

 

Barbas Ε. 2002 Douglas fir (Pseudotsuga menziesii mirb.) and walnut (Juglans nigra x Juglans regia) juvenility biochemical markers in in vitro culture.  Proccedings of the  10th congress of the Hellenic scientific society of forestry, 26-29 may, Tripolis, p. 198-205

 

Barbas E. 2002 Effects of a double phase media system on growth and mineral content of in vitro walnut explants (Juglans nigra x Juglans regia). Proceedings of the 9th Hellenic Scientific Society of Plant Breeding, 30 October - 1 November 2002, Thessaloniki, p. 408-414

 

Hibrand-Saint Oyant L., Barbas E., Rajapakse S., Crespel L. και Foucher F. 2004 Development of a rose genetic map with SSR markers. Proceedings of the 10th Hellenic Scientific Society of Plant Breeding, Athens, 24-26 November, p. 332-336.

 

Orfanoudakis M., Papaioannou A., Barbas E.  2004 Preliminary studies in Αlnus glutinosa root symbiosis in the field. EUROSOIL 2004, 04 – 12 September, Freiburg

 

Pernet A., Barbas E. Peltier D. 2004 Molecular survey of the genetic diversity from commercial Pelrgonium x Hortorum cultivars compared to botanical accessions. Genetic variation for Plant breeding, Proceedings Of The 17th Eucarpia General Congress 8 - 11 September 2004, Tulln – Austria, Eds Vollmann, Grausgruber and Ruckenbauer, Publisher BOKU – University of Natural Resources and Applied, Life Sciences, Vienna, Austria, poster,  p.171

 

Strullu D. G., Ledon V., Voets L., Barbas E., Simo P., Boccon-Gibod J. 2004 Study of the mycotrophy in vitro of a somatic hybrid of potato in association with Glomus intraradices. Fifth IVCHB Symbosium, In vitro culture and horticultural breeding, Debrecen, Hungary, September 12-17, poster.

 

Pavlidis P., Barbas E. and Vasilakakis M. 2006 Effect of substrate, growth regulators and auxin overproducing Hebeloma cylindrosporum strains in rooting of two quince clones (EM A and BA 29) microshoots. Fifth IVCHB Symposium, In vitro culture and horticultural breeding, DEBRECEN, Hungary, September 12-17  Acta Hort. (ISHS) 725:271-278

 

Strullu D.G., Voets L., Siham M., Ledon V. Barbas E. and Declerck S. 2005 Mycorhization de la pomme de terre : stratégies d’inoculation et technologies d’encapsulation. Les 3emes rencontres du Végétal en Horticulture, Semences et Paysage. Angers, France, σ. 69-70.

Τchatchoua T. D., Barbas E. and  Aravanopoulos P. 2007.Micropropagation of Castanea sativa (MILL) selected trees. Proceedings of the 13thCongress of the Hellenic Scientific Society of Forestry, 7-10 October 2007, Kastoria, σ. 391-399

Barbas E. and Tziertzidou K. 2008 Influence of mycorrhizal synthesis during in vitro development of Alnus glutinosa (L.) GAERTN. plants. Propagation of ornamental plants 8 (4) : 215-217

 

Doussa V. and Barbas Ε. 2009 Artificial mycorrhizal synthesis between the species Quercus pubescens Willd, Quercus coccifera L. και Castanea sativa Mill. and ascomycetes of the genus Tuber (T. melanosporum  Vitt.  and T. aestivum Vitt. ) Proceedings of the 13th Congress of the Hellenic scientific society of forestry, 1-4 November 2007, Patras, (sous press).

 

Gougoutsa C. and Barbas E. (2010) Micropropagation of hazelnut (Corylus avellana). Proceedings of the 13th Congress of the Hellenic Scientific Society of Plant Breeding, October 2010,Kalamata, (sous press)

 

European Union Cost Meeting Presentations:

 

Strullu D.G., Barbas E.  and Strullu-Derrien C. 2008 AM FUNGI IN PLANTA: 20 years of quality progress of in vitro culture. Mycorrhiza COST action 870 meeting 17-19 sept.08, Thessaloniki, Greece

 

Barbas E. and Tziertzidou K. 2006 Influence of the mycorrhizal fungus Paxillus involutus on the in vitro development of Alnus glutinosa explants. Genosilva : European Forest Genomics Network COST  E-28 action meeting, 2-3 feb. 06, Alcalá de Henares, Madrid, Spain

 

Patent:

(WO/2005/000008) METHOD FOR IN VITRO PRODUCTION OF MYCORRHIZAL FUNGI MYCOCALLUS AND MYCORRHIZED BIOLOGICAL SUPPORT OBTAINED THUS

 
 

Pub. No.:

  

WO/2005/000008

  

International Application No.:

  

PCT/FR2004/001570

Publication Date:

06.01.2005

International Filing Date:

23.06.2004

IPC:

A01G 1/04 (2006.01), C12N 1/14 (2006.01)

Applicants:

UNIVERSITE D'ANGERS [FR/FR]; 40, rue de Rennes, F-49000 Angers (FR) (All Except US).
STRULLU, Désiré-Georges [FR/FR]; (FR) (US Only).
BARBAS, Evangelos
 [FR/FR]; (FR) (US Only).

Inventors:

STRULLU, Désiré-Georges; (FR).
BARBAS, Evangelos; (FR).

Agent:

COURMARIE, Michèle; Cabinet Brema, 78, avenue Raymond Poincaré, F-75116 Paris (FR) .

Priority Data:

03/07596

 

24.06.2003

 

FR

Title:

(EN) METHOD FOR IN VITRO PRODUCTION OF MYCORRHIZAL FUNGI MYCOCALLUS AND MYCORRHIZED BIOLOGICAL SUPPORT OBTAINED THUS

(FR) PROCEDE DE PRODUCTION IN VITRO DE CHAMPIGNONS MYCORHIZIENS, MYCOCAL ET SUPPORT BIOLOGIQUE MYCORHIZE OBTENU

Abstract:

(EN) The invention relates to an in vitro production method in monoxenic conditions of mycorrhizal fungi, preferably of the order Glomales, in particular the genus Glomus, from an inoculant comprising Mycocallusmycorrhizae and/or vesicles and/or spores obtained from at least one fragment of mycelium and/or mycelium from mycorrhizal fungi. The method is characterised in comprising at least one step for culture of the inoculant on a nutrient medium in the presence of at least one rhizogenic callus from at least one mycorrhizogenic vegetable. The above is of application as a substitute for chemical fertilization, diagnostic tests, biological extracts, DNA and genomics.

(FR) L'invention concerne un procédé de production in vitro, en condition monoxénique, de champignons mycorhiziens, de préférence de l'ordre des Glomales, en particulier du genre Glomus, à partir d'un inoculum constitué de mycorhizes et/ou de vésicules et/ou de spores pourvues d'au moins un fragment de mycélium et/ou de mycélium, de champignons mycorhiziens. Ce procédé est caractérisé en ce qu'il comporte au moins une étape de culture de l'inoculum sur un milieu nutritif en présence d'au moins un cal rhizogène issu d'au moins un végétal mycorhizogène. Application : substitut d'engrais chimique. Tests de diagnostics. Extraits biologiques. ADN et génomique.

Designated States:

AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Org. (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (EAPO) (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IT, LU, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (OAPI) (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).

Publication Language:

French (FR)

Filing Language:

French (FR)

 

Languages :             Greek, French, English

 

Software:                 Microsoft office, Statitcf, Statgraphics, SPSS, Corel draw, Photoshop

 

 



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