The Laboratory of Professor Costas Triantaphyllidis

 

The Laboratory of Professor Costas Triantaphyllidis is engaged in a wide range of innovative research projects related to morphological, cytogenetic, biochemical and DNA analysis of various animal species. The laboratory has almost 30 years experience in analysing the genetic composition of numerous animal groups (i.e. fish, Artemia, mammals, insects, humans) using advanced genetic techniques:

• Nuclear markers: Allozymes and direct DNA approaches (SSCP analysis, RAPDs, mini- and macro-satellites, DNA sequencing).
• Mitochondrial DNA markers: RFLPs with or without sequencing.

 

Much of the work has focused on the population genetics and the molecular genetics of fish (Salmo trutta, Silurus glanis, Mullus barbatus, Leuciscus cephalus, Trachurus trachurus) and other animal species (Mytilus galoprovincialis, European lobster). Study of human populations involves the analysis of the genetic composition of Greeks (mtDNA, autosomal microsatellites and Y-linked markers), the investigation of common diseases (cystic fibrosis, tuberous sclerosis, thalassaemia and diabetes), and the application of genetics in medico-legal cases.

 

    The research Team directed by Prof. Costas Triantaphyllidis works in fish and human genetics since 1980. The Lab has established an excellent record of co-ordination of international projects with many and varied laboratories across Europe. Professor C. Triantaphyllidis has successfully led, as Scientist in charge, several research projects (EU funded, National Greek projects, international bilateral projects and NATO). He is currently a partner in two EU-funded project (QLK5-CT1999-01157) concerning the “Genetic identification of fish eggs by species specific DNA markers for use in stock biomass assessments and detection of commercial fraud”, and (QLRT-2001-01302) concerning the “Genetic implications in the production of rotifers in commercial hatcheries” and one National project.

 

Publications

 

 

Apostolidis A., Z. Mamuris and C. Triantaphyllidis  (2001). Phylogenetic relationships among four species of Mullidae family (Perciformes) inferred from DNA sequences of mitochondrial cytochrome b and 16 S rRNA genes. Bioch.  Syst. Ecol. 29: 901-909.

 

Αpostolidis A., Karakousis Y. and C. Triantaphyllidis (1996). Genetic divergence and phylogenetic relationships among brown trout (Salmo trutta L.) populations from Greece and other European countries. Heredity 76: 551-560.

 

Apostolidis A., Karakousis Y., and C. Triantaphyllidis (1996). Genetic differentiation and phylogenetic relationships among Greek brown trout (Salmo trutta L) populations as revealed by RFLP analysis of PCR amplified mitochondrial DNA segments. Heredity 77: 608-618

 

Apostolidis A., C. Triantaphyllidis, A. Kouvatsi and P.S. Economidis (1997). Mitochondrial DNA sequences variation and phylogeography among Salmo trutta L. (Greek brown trout) populations. Molecular Ecology 6: 531:542.

 

Gollman G., Y. Bouvet, Y. Karakousis and C. Triantaphyllidis (1997). Genetic variability in Chondrostoma from Austrian, French and Greek rivers (Teleostei, Cyprinidae). J. Zool. Syst. Zool. Research 35: 165-169.

 

Imsiridou A., Y. Karakousis and C. Triantaphyllidis (1997). Genetic polymorphism and differentiation among chub Leuciscus cephalus (Pisces, Cyprinidae) populations of Greece. Bioch. Syst. Ecol. 25: 537-546.

 

Imsiridou A., Apostolidis A., Durand J., Briolay J., Bouvet Y. and C. Triantaphyllidis (1998). Genetic differentiation and phylogenetic relationships among Greek Chub Leuciscus cephalus L. (Pisces, Cyprinidae) populations as revealed by RFLP analysis of amplified mitochondrial DNA segments. Bioch. Syst. Ecol. 26: 415-429.

 

Imsiridou A. & C. Triantaphyllidis (2001). Use of allozyme electrophoresis for   differentiation between two European

           hake Merluccius   merluccius L. (Gadidae) populations ).  Biologia, Bratislava  56: 545-547.

 

Kondopoulou H., R. Loftus, A. Kouvatsi and C. Triantaphyllidis (1999). Genetic studies in five Greek population samples using 12 highly polymorphic DNA loci. Human Biology 71: 27-42.

 

Kondopoulou H., A. Kouvatsi and C. Triantaphyllidis (2001). Forensic evaluation of 12 variable number of tandem

     repeat (VNTR) loci in the Greek population.   Forensic Science Inter. 124: 228-230.

 

Krieg F., Estoup A., Triantafyllidis A. and Guyomard R. (1999). Isolation of microsatellite loci in European catfish, Silurus glanis. Molecular Ecology 8: 1964-1967.

 

Krieg F., Triantafyllidis A. and Guyomard R. (1999). Mitochondrial DNA variation in European populations of Silurus glanis. J. Fish Biol. 56: 713-724.

 

Laikre L. A. Antunes, A. Apostolidis, …….… and C. Triantaphyllidis (1999).                                    

      Conservation  genetic management of brown trout (Salmo   trutta) in Europe , ISBN 87-987732-0-8 1999, pages 92.

 

Mamuris   Z.,  Apostolidis   A.,  Theodorou  A. J.,  Triantaphyllidis  C. (1998). Application of random amplified polymorphic DNA (RAPD) markers to evaluate intraspecific genetic variation in red mullet (Mullus barbatus). Marine Biology 132: 171-178.

 

Mamuris Z., C. Stamatis, K. Moutou, Apostolidis A. and C. Triantaphyllidis (2001). Application of RFLP analysis of      

          PCR amplified  mitochondrial DNA sequences to evaluate genetic variation in striped red mullet (Mullus

          surmuletus L) and in red mullet (Mullus barbatus L.) populations. Mar. Biotechn. 3, 264-274.

 

Mamouris Z., Costas Stamatis, M. Bani and C. Triantaphyllidis (1999a). Taxonomic relationships between four species of the Mullidae family revealed by three genetic methods: allozymes, random amplified polymorphic DNA and mitochondrial DNA.  J. Fish Biol. 55:572-587.

 

Mamuris Z., Stamatis C., Triantaphyllidis C. (1999b). Intraspecific genetic variation of striped red mullet (Mullus surmuletus, L.) in the Mediterranean Sea assessed by allozyme and Random Amplified Polymorphic DNA (RAPD) analysis. Heredity 83: 30-38.

 

Martin Richards, V. Macaulay,  …  Costas Triantaphyllidis et al. (2000). Tracing European founder lineages in the Near Eastern mtDNA pool. Am. J. Hum. Genet. 67:1251-1276.

 

Triantafyllidis A., Abatzopoulos T.J. and Economidis P.S. (1999). Genetic differentiation and phylogenetic relationships among Greek Silurus glanis and Silurus aristotelis (Pisces, Siluridae) populations, assessed by PCR-RFLP analysis of mitochondrial DNA segments. Heredity 82, 503-509.