|
We utilise UPLC in order to exploit it superior
separation power.
UPLC is combined with a hybrid QTOF (Waters Q micro) and a hybrid triple quadropole linear ion trap (Q 4000, API SCIEX). For more specialised analysis, data is acquired in Orbi Trap MS (Thermo), Agilent TOF-MS or the QTOF premier (Waters). GC-MS analysis is done in a Waters GC-TOF-MS. NMR spectroscopy is performed on a Bruker 600 MHz. LC-MS data is treated with a variety of specially developed software tools for peak finding and alignment: MarkerView, MarkerLynx, MZMine, Mass Profiler. The generated peak tables are analysed in PCA and then also in PLS-DA using SIMCA P11. A typical workflow of our research is given here.
|
