FP6 TOK-IAP (MTKI 029640-2005) “Biomarker Discovery” (2006-2008)

FP7 ERG 202132 “Advanced Bioanalytical Technologies for systems biology Studies” (2008-2011)

Metabonomic/Metabolomic Research

Here are some posters (pdf format) presented by the group at conferences such as:

• The LC-MS Montreaux 2006 Conference
• The HPLC 2007 conference (June 2007)
• The 3rd Metabolomics society conference (June 2007)
• The Guildford Bioanalytical Symposium (2007)
• IMA2007, ISC 2008 and so on.

Publications

“Within-day reproducibility of an HPLC-MS-Based method for metabonomic analysis: Application to human urine”. Gika HG, Theodoridis GA, Wingate JE, Wilson Ian D.
Journal of Proteome Research 6 (8) 2007, 3291-3303    

“Liquid Chromatography and Ultra Performance Liquid Chromatography-Mass Spectrometry fingerprinting of human urine: Sample stability under different handling and storage conditions for metabonomics studies”. H.G. Gika, G. A. Theodoridis, I D. Wilson
J. Chromatography A, 1189, 2008, 314-322

“LC-MS-based methodology for global metabolite profiling in metabonomics/metabolomics” Georgios Theodoridis, Helen Gika, Ian D. Wilson
TrAC Trends in Analytical Chemistry, 27, 2008, 251-260 

“Hydrophilic Interaction and Reversed-phase Liquid Chromatography for Metabonomic Analysis of Zucker Rat Urine Samples by Ultra Performance Liquid Chromatography Time of Flight Mass Spectrometry”. Helen G. Gika, Georgios A. Theodoridis, Ian D. Wilson
Journal of Separation Science, 31, 2008, 1598-1608  

“Hyphenated techniques for global metabolite profiling” Editorial Georgios Theodoridis, Ian D. Wilson
J. Chromatogr. B, 871, 2008, 141-142    

“High Temperature-Ultra Performance Liquid Chromatography Mass Spectrometry for the Metabonomic Analysis of Zucker rat urine”. Helen G. Gika, Georgios Theodoridis, Jon Extance, Antony M. Edge, Ian D.Wilson
J. Chromatogr. B, 871, 2008, 279-287    

“Profiling and Biomarker Identification in plasma from different Zucker rat strains via high mass accuracy MSn analysis using LC-MS with a quadrupole ion trap-time of flight MS” Neil Loftus, Kozo Miseki, Junko Iida, Helen G Gika, Georgios Theodoridis, Ian D Wilson
Rapid Communications in Mass Spectrometry 22 (16), 2008, 2547-2554  

“Evaluation of the Repeatability Reproducibility of Ultra-Performance Liquid Chromatography UPLC-TOF- MS Global Metabolic Profiling of Human Urine samples”. Helen G. Gika, Euan Macpherson, Georgios Theodoridis, Ian D. Wilson,
J. Chromatogr. B, 871, 2008, 299-305

Presentations

• 23rd LC/MS Symposium on LC/MS, November 8-10, 2006 Montrex.
  Posters:
  1. A quality Control approach for LC-MS based Metabonomic Analysis
  2. A comparison of UPLC-MS platforms in Metabonomic Analysis of human urine samples
• Metabomeeting 3: A two day meeting on metabolic profiling, December 2006, Imperial College London.
  Lecture: METABOLOMICS AND LC-MS: MS SPECTROMETRY NOT MASS SPECULATION.  
• 55th ASMS Conference on Mass Spectrometry, 3-7 June 2007, Indianapolis
  Poster: Global Metabolite Profiling of Human Urine using LC-MS on a new Ion-Trap Time of Flight hybrid Mass Spectrometer, H. Gika, G. Theodoridis, I. Wilson, N. Loftus  
• Metabolomics society 3rd Annual conference, 11-14 June 2007 Manchester.
  Posters:
  1. METHODOLOGY FOR THE SIMULTANEOUS, UNBIASED, COMPARISON OF TWO DIFFERENT MASS SPECTROMETERS FOR LC-MS-BASED METABONOMIC ANALYSIS
  2. A SYSTEMS BIOLOGY STUDY OF MICE RAISED IN DIFFERENT ENVIRONMENTS: INVESTIGATION OF THE EFFECT OF GUT MICROFLORA AND THE GLOBAL METABOLOME, H. Gika et al
  3. METABOLOMICS ANALYSIS OF BLOOD PLASMA FROM STATIN-TREATED RATS BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY. GAINING INSIGHT ON STATIN INDUCED MYOPATHY
  4. QUALITY CONTROL IN LC-MS METABONOMIC ANALYSIS. APPROACHES, ISSUES, GUIDELINES
  5. UPLC-MS BASED METABONOMIC ANALYSIS OF BLOOD PLASMA FROM MICE REARED ON DIFFERENT DIET, H.Gika
  6. HOW STABLE ARE THE URINE SAMPLES STORED IN YOUR FREEZER? AN LC-MS METABOLOMICS STUDY, H. Gika  
• 31st International Symposium on High Performance Liquid Phase Separations HPLC 2007, 17-21 June, Ghent.
  Oral presentation: "HIGH PERFORMANCE SEPARATIONS FOR HIGH RISE QUESTS: LC-MS BASED GLOBAL METABOLITE PROFILING FOR BIOMARKER DISCOVERY"
  Posters:
  1. COMPARISON OF UPLC-MS VS HPLC-MS IN SAMPLE FINGERPRINTING FOR METABOLOMICS RESEARCH. IS THERE A REAL NICHE FOR UPLC?
  2. A SYSTEMS BIOLOGY STUDY OF MICE RAISED IN DIFFERENT ENVIRONMENTS: INVESTIGATION OF THE EFFECT OF GUT MICROFLORA AND THE GLOBAL METABOLOME, H. Gika et al
  3. METABOLOMICS ANALYSIS OF BLOOD PLASMA FROM STATIN-TREATED RATS BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY. GAINING INSIGHT ON STATIN INDUCED MYOPATHY.
  4. QUALITY CONTROL IN LC-MS METABONOMIC ANALYSIS. APPROACHES, ISSUES, GUIDELINES,
  5. UPLC-MS BASED METABONOMIC ANALYSIS OF BLOOD PLASMA FROM MICE REARED ON DIFFERENT DIET, H. Gika
  6. HOW STABLE ARE THE URINE SAMPLES STORED IN YOUR FREEZER? AN LC-MS METABOLOMICS STUDY, H. Gika  
• 17th International Reid Bioanalytical Forum, 9-12 July 2007, Guidford, UK
  Invited lecture: HYPHENATED MASS SPECTROMETRY TECHNOLOGIES FOR GLOBAL METABOLITE PROFILING. A HAPPY MARRIAGE?  
• International Conference on Instrumental Methods of Analysis ΙΜΑ2007, Patras, October 2007,
  Lecture: HYPHENATED MASS SPECTROMETRY TECHNOLOGIES FOR GLOBAL METABOLITE PROFILING. A HAPPY MARRIAGE?
  Posters:
  1. A SYSTEMS BIOLOGY STUDY OF MICE RAISED IN DIFFERENT ENVIRONMENTS: INVESTIGATION OF THE EFFECT OF GUT MICROFLORA AND THE GLOBAL METABOLOME, Gika et al
  2. METABOLOMICS ANALYSIS OF BLOOD PLASMA FROM STATIN-TREATED RATS BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY. GAINING INSIGHT ON STATIN INDUCED MYOPATHY.
  3. UPLC-MS BASED METABONOMIC ANALYSIS OF BLOOD PLASMA FROM MICE REARED ON DIFFERENT DIET, Gika et al  
• 24th Montreux Symposium on LC/MS & Related Techniques October 10-12, 2007 Hilton Head Island, South Carolina, USA
  Poster: "PROFILING AND BIOMARKER IDENTIFICATION IN BIOLOGICAL FLUIDS FROM DIFFERING ZUCKER RAT STRAINS USING HIGH MASS ACCURACY MSN ANALYSIS", H. Gika, G. Theodoridis, I. Wilson, N. Loftus et. al.  
• Session Sampling strategy, TOOLS AND METHODS FOR MASS SPECTROMETRY METABOLOMICS IN NUTRITION, NUGO , INRA Clermont-Ferrand, France December 12-14, 2007, H. Gika, G. Theodoridis
  Moderators (invited by the organisers).  
• 4th Metabomeeting Lyon France 28-29April 2008.
  Lecture: "ASSESSMENT OF REPRODUCIBILITY AND ROBUSTNESS OF UPLC-MS DATA FOR THE METABOLOMIC STUDY OF HUMAN SERUM", Eva Zelena et al,  
• 56th ASMS Conference on Mass Spectrometry June 1 - 5, 2008μ Colorado Convention Center, Denver, Colorado
  Poster: "IDENTIFYING BIOMARKERS FOR RHEUMATIOD ARTHRITIS IN THE HUMAN TNF-DRIVEN TG197 MOUSE MODEL USING HIGH MASS ACCURACY MSn ANALYSIS". Poster presentation in session Metabolomics II Eleni Gika; Georgios Theodoridis; NEIL J LOFTUS; et.al.  
• Genomes to Systems Conference. Manchester, UK, 17-19 March 2008
  Poster: "ASSESSMENT OF THE REPRODUCIBILITY AND ROBUSTNESS OF UPLC-TOF-MS METABOLOMIC DATA ACQUIRED FROM HUMAN SERUM IN THE HUSERMET PROJECT". Eva Zelena, Warwick B. Dunn, David Broadhurst, Sue McIntyre, Kathleen Carroll, Eleni Gika, Georgios Theodoridis, Antony Halsall, Ian D Willson & Douglas B. Kell.  
• 27th  International Symposium on Chromatography SC, Munster September 2008,
  Posters:
  1. "Assessing the sensitivity of data processing softwares for Global metabolite profiling-metabonomic analysis of urine and plasma samples". A. Pechlivanis, F. Michopoulos, H. Gika, G. Theodoridis, I.D. Wilson
  2. "Effect of sample preparation in LC-MS based Global metabolic profiling" F. Michopoulos, A. Pechlivanis, H. Gika, G. Theodoridis, I.D. Wilson