Effects of short term exersise on levels of TAT and PAP.

Effects of short term exercise on levels of TAT and PAP.

Mavrovouniotis F, Foka Z, Mavrovouniotis H, Pithara, E, Xanthopoulos V, Gerotziafas G, Makris PE.

Haem & Thrombosis Unit, AHEPA Univ. Hospital, Thessaloniki, GREECE

In order to check the effect of short term exercise on levels of TAT and PAP, we studied two groups of healthy subjects. Material: 38 subjects divided into two groups were studied. Control group: 20 individuals who were not athletes, aged 36,32± 4,45 years old, were submitted to aerobic exercise for 21 min. Study group: 18 athletes aged 22±1,62 years old were submitted to the same protocol.. Methods: We had blood draw before and immediately after the exercise. We determined the levels of TAT and PAP using an immunoenzymatic method (Behring). Results: The results are presented in the following table. The comparison of these mean values with the t-test has shown that there is a difference in the levels of PAP and TAT in the control group. Concerning the group of athletes there was observed a very significant difference only in the levels of PAP. In order to establish our results we applied the x²test as a quality criterion. This test showed a significant difference only in the levels of PAP mainly in the athletes group.

Control group Athletes

TAT μg/l PAPμg/l TATμg/l PAPμg/l

before 44,7±28,1 45,0±35,8 49,7±48,1 54,4± 60,4

after 28,8±12,7 342,5±634,6 33,5±22,5 144,4±163,4

Comments. There is a similar response to the short term exercise (triggering of the fibrinolysis) for the two groups, but more pronounced in the group of athletes.

FACTOR V-LEIDEN IN GREEK THROMBOPHILIC PATIENTS.

II- Relationship with aPC-resistAnce test.

Lambropoulos AF, Foka Z, Kotsis A, Makris PE.

Biology Dep & Haem Thromb. Unit AHEPA Hospital, Aristotelian University of Thessaloniki, Greece

Materials and Methods.

We examined 106 patients with unexplained thrombophilic tendency, aged 5-73 years old (average 45±12,29). 17 patients among them were members of the families of the propositi. We also examined 45 healthy males aged 29,1 ± 8,1 years old as a control group.

Methods: We used: a) for the aPC-r test the Coatest kit (Chromogenix AB) modified by the addition of factor V deficient plasma. b) a PCR based assay (followed by a cleavage by the restriction enzyme Mnl1 and electrophoresis in agarose gel 5%), for the detection of mutated factor V Leiden.

Results: 1) With the application of PCR based technique we found 33 heterozygotes and 2 homozygotes for this point mutation, or 33,3%. 22 patients in 35 (21 heterozygotes and 1 homozygote) were isolated, as the other family members were not available for testing. The rest 13 of them belonged to 4 families (12 heterozygotes and 1 homozygote). Therefore, we discovered 22 patients in 93 with this mutation, or 23,6%. 2). According to our reference limits for the aPC-r test (X-2SD=3,42±2(0,56)=2,3, CI=95%) we found only 40 patients out of 106 below this value (20 heterozygotes, 2 homozygotes and 20 patients without the mutation). 3). We also found 1 apparently healthy individual out of 45 with this m;utation (2,2%).

Conclusion- comments:. 1). The prevalence of the mutation Arg⁵⁰⁶®Gln among Greek patients with a thrombophilic tendency is amounted to 23,6% in isolated patients and 33,3% in all tested patients. It is a quite high percentage and it is in agreement with the literature. 2) It is interesting that only 1 healthy subject out of 45 (2,2%) had the mutation. 3) There was incomplete cosegregation of heterozygosity for the Arg⁵⁰⁶®Gln mutation with aPC-r test, showing that the functional assay alone is not sufficient for a firm diagnosis.

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FACTOR V-LEIDEN IN GREEK THROMBOPHILIC PATIENTS. II- Relationship with aPC-resistance test. Lambropoulos AF, Foka Z, Kotsis A, Makris PE.

Biology Dep & Haem Thromb. Unit AHEPA University Hospital, Thessaloniki, Greece.

The aim of this study was to investigate the unexplained thrombotic tendency of Greek patients who had had one or more episodes of superficial or DVT with or without pulmonary embolism at an age less than 40 years. Factor V Leiden is the most frequent cause of thrombophilia. It is also believed that factor V Leiden is strongly correlated with the resistance to activated protein C (aPC-r). We checked the concordance of a pathological aPC-r test with the factor V Leiden mutation. Materials and Methods. We examined 106 patients with unexplained thrombophilic tendency, aged 5-73 years old (average 45±12,29). 17 patients among them were members of the families of the propositi. We also examined 45 healthy males aged 29,1 ± 8,1 years old as a control group. Methods: We used: a) for the aPC-r test the Coatest kit (Chromogenix AB) modified by the addition of factor V deficient plasma. b) a PCR based assay (followed by a cleavage by the restriction enzyme Mnl1 and electrophoresis in agarose gel 5%), for the detection of mutated factor V Leiden. Results: 1) With the application of PCR based technique we found 33 heterozygotes and 2 homozygotes for this point mutation, or 33,3%. 22 patients in 35 (21 heterozygotes and 1 homozygote) were isolated, as the other family members were not available for testing. The rest 13 of them belonged to 4 families (12 heterozygotes and 1 homozygote). Therefore, we discovered 22 patients in 93 with this mutation, or 23,6%. 2). According to our reference limits for the aPC-r test (X-2SD=3,42±2(0,56)=2,3, CI=95%) we found only 40 patients out of 106 below this value (20 heterozygotes, 2 homozygotes and 20 patients without the mutation). 3). We also found 1 apparently healthy individual out of 45 with this m;utation (2,2%).