Nikolakaki Eleni, Assist. Professor
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PERSONAL
Date of Birth:
March
20, 1962
Marital status:
Married
with two children
Country of
citizenchip:
Languages:
Greek
(mother tongue), English (fluent)
EDUCATION
1979 - 1984 Attended Aristotelian University of
Thessaloniki, awarded first degree in Chemistry.
1984 - 1990 Graduate student in the Laboratory of Biochemistry, Department of Chemistry,
Aristotelian University of Thessaloniki
Title
of PhD thesis “Phosphoryation in a
cell-free system from mouse liver -
Purification and characterization of a novel protein kinase regulated by cyclic
nucleotides”
June
1, 1989 - October 10, 1989 Visiting researcher in
Dr. Woodgett’s
laboratory, Ludwig Institute for Cancer Research,
January 1991 - December 1992 Post-doctoral fellow in Dr. Woodgett’s laboratory, Ludwig Institute
for Cancer Research,
1994 – 1996 Three
short visits (1-2 months each) in Dr. S. Georgatos’ laboratory, European Molecular Biology Laboratory (EMBL),
POSITIONS
1991 - 1999
Researcher (ΠΕ/classification Chemist) in the Laboratory of Biochemistry, Department of Chemistry, Aristotelian
University of Thessaloniki.
1999 - 2004 Lecturer
in the Laboratory of Biochemistry, Department of Chemistry,
2004 – present Assistant Professor in the
Laboratory of Biochemistry, Department of Chemistry, Aristotelian
University of Thessaloniki.
AWARDS -
FELLOWSHIPS
June 1989 - October 1989 Short-term
EMBO fellowsip
January 1991 - December 1992 Post-doctoral
fellowship from Ludwig Institute for Cancer Research,
TEACHING
More than 10 years of Biochemistry and Molecular Biology courses
and laboratories for undergraduate and graduate students of the Chemistry,
Pharmaceutical and Biological Department of the Aristotelian University of Thessaloniki
STUDENT THESIS SUPERVISION
More than 10 undergraduate,
SCIENTIFIC SOCIETIES
o British
Biochemical Society
o Hellenic Society
of Biochemistry and Molecular Biology
o Hellenic
Chemical Society
SCIENTIFIC ACTIVITY
o 28 publications in peer-reviewed
journals
o More than 4000 non-self
citations, H-index 18, Total impact factor over 150, average 5.4
o 35 international and national
presentations, 3 invited lectures.
FUNDING
Coordinator
of the following Research Grants
o 2011 - 2014 Program for basic research financed by the Greek
Ministry of Education and the European Community (HPAKΛEITOΣ), entitled
“Regulation of differentiation of human erythroleukaemic cells by SRPK1 –
Development of specific inhibitors” (45,000 €).
Participant in the following Research Grants
o 1994 -1996 Greek-German bilateral cooperation financed by the
General Secretariat for Research & Technology, entitled “Purification,
characterization and cloning of the protein kinase that was found associated in
vivo with the lamin B receptor” (4,000,000 dr).
o 1996 - 1998 Program for
scientific research financed by the General Secretariat for Research &
Technology (ΠENEΔ), entitled “Modulation of the biological activity of lamin B
receptor by a novel serine/threonine protein kinase - Cloning and
characterization of this kinase” (8,000,000 dr).
o 1998 - 2000 Program for scientific research financed by the
National Health Council (KEΣΥ), entitled
“Chromatin condensation during spermatogenesis” (2,000,000 dr).
o 1999 - 2001 Program for scientific research financed by the General
Secretariat for Research & Technology (ΠENEΔ), entitled “Self-assembly of
the nuclear envelope - Mechanism, regulation and development of inhibitors” (50,000,000 dr).
o 2001 - 2003 Greek-French bilateral cooperation financed by the
General Secretariat for Research & Technology, entitled “Characterization of SRPK and
LAMMER protein kinases” (4,200,000 dr).
o 2001 - 2004 Program for
scientific research financed by the General Secretariat for Research &
Technology (ΠENEΔ), entitled “Heterochromatin protein 1 (HP1) as a molecular
marker in cancer” (40,000,000 dr).
o 2003 - 2005 Program for basic research financed by the Greek
Ministry of Education and the European Community (HPAKΛEITOΣ), entitled
“Biological function and protein interactions of SRPK1” (35,000 €).
o 2005 - 2007 Program for basic research financed by the Greek
Ministry of Education and the European Community (ΠΥTHAGOPAS II), entitled
“Control of differentiation and apoptosis of human erythroleukaemic cells by
SRPK1 - Subcellular distibution of the kinase” (40,000 €).
COLLABORATIONS
o
o
o
o Medical Scool,
o Université Paris
11, CNRS Orsay, France (Dr. L. Rabinow)
o Dana Farber Cancer Institute,
o Department of Pharmacology,
o Laboratory of Statistical Genetics, The Rockefeller
o Laboratory of Experimental Hematology, Faculty of
Medicine,
o Department of Obstetrics and Gynecology, Medical
o Applied Biosystems,
CURRENT RESEARCH INTERESTS
o Protein phosphorylation and signal transduction
o Biochemical properties, function and regulation of
protein kinases phosphorylating Serine/Arginine(SR) motifs
o Molecular mechanisms regulating differentiation
of human erythroleukaemic and erythroid progenitor cells
PUBLICATIONS (in peer reviewed journals)
1. Giannakouros, T., Nikolakaki,
E., and Georgatsos, J.G. (1990) Concentration dependent effects of natural polyamines on peptide chain initiation and elongation in a cell-free system of protein
synthesis. Mol.
Cell. Biochem. 99,
9-19.
2. Nikolakaki, E., Giannakouros,
T., and Georgatsos, J.G. (1990) Inhibition of protein synthesis by acetyl-coenzyme A in a cell-free
system. Possible involvment
of acetylation in the regulation of translation. Biofactors 2,
255-258.
3. Pulverer, B.J., Kyriakis, J.M., Avruch, J.,
Nikolakaki, E., and Woodgett, J.R. (1991) Phosphorylation of c-jun mediated by MAP
kinases. Nature
353, 670-674.
4. Plyte, S.E., Hughes, K., Nikolakaki, E.,
Pulverer, B.J., and Woogdett, J.R. (1992) Glycogen synthase kinase-3: function in
oncogenesis and development. Biochem. Biophys. Acta 1114, 147-162.
5. Hughes, K., Nikolakaki,
E., Plyte, S.E., Totty, N.F., and Woodgett, J.R. (1993) Modulation of the glycogen synthase kinase-3
family by phosphorylation. EMBO J. 12, 803-808.
6. Nikolakaki, E.,
Coffer, P.J., Hemelsoet, R., Woodgett, J.R., and Defize, L.H.K.
(1993) Glycogen synthase kinase-3
phosphorylates jun-family members in vitro and negatively regulates their transcriptional potential in intact cells. Oncogene 8, 833-840.
7. Woodgett, J.R.,
Pulverer, B.J., Nikolakaki, E., Plyte, S.E., Hughes, K., Franklin, C.C., and Kraft, A.S. (1993) Regulation of Jun/AP-1 oncoproteins by protein phosphorylation. Adv.
Second Messenger Prot. Phosph. Res. 28,
261-269.
8. Kyriakis, J.M.,
Banerjee, P., Nikolakaki, E., Dai, T., Rubie, E.A., Ahmad, M.F., Avruch, J., and Woodgett, J.R.
(1994) The stress-activated protein
kinase subfamily of c-Jun
kinases. Nature 369, 156-160.
9. Woodgett, J.R., Pulverer, B.J., Plyte, S.E., Hughes, K., and Nikolakaki, E. (1994) Nuclear onco-protein targets
of signal transduction pathways. Pigment
Cell
Res. 7, 96-100.
10. Nikolakaki, E.,
Simos G., Georgatos S.D., and Giannakouros, T.
(1996) A nuclear envelope-bound kinase phosphorylates arginine-serine motifs and modulates interactions between the lamin
B receptor and other nuclear proteins. J. Biol. Chem.
271, 8365-8372.
11. Nikolakaki, E.,
Meier, J., Simos, G., Georgatos, S.D., and Giannakouros, T. (1997) Mitotic phosphorylation of
the lamin B receptor (LBR) by a serine/arginine kinase and p34cdc2. J.
Biol. Chem. 272, 6208-6213.
12. Papoutsopoulou, S., Nikolakaki,
E., and Giannakouros, T. (1999) SRPK1 and LBR protein kinases show identical substrate
specificities. Biochem. Biophys. Res.
Commun. 255, 602-607.
13. Nikolakaki, E., Fissentzidis, A.,
Giannakouros, T., and Georgatsos J.G.
(1999) Purification
and characterization of a dimer form of the cAMP-dependent protein kinase from
mouse liver cytosol. Mol.
Cell. Biochem. 197, 117-128.
14. Papoutsopoulou, S., Nikolakaki,
E., Chalepakis, G., Kruft, V., Chevaillier, P., and Giannakouros, T. (1999)
SR protein-specific
kinase 1 is highly expressed in testis
and phosphorylates protamine 1.
Nucleic Acids Res. 27, 2972-2980.
15. Hartmann, A., Rujescu, D., Giannakouros, T., Nikolakaki, E., Goedert, M., Mandelkow,
E., Gao, Q.S., Andreadis, A., and Stamm, S. (2001) Regulation of alternative
splicing of human tau exon 10 by phosphorylation of splicing factors. Mol. Cell. Neurosci. 18,
80-90.
16. Nikolakaki,
E., Kohen, R., Hartmann, A.M., Stamm, S., Georgatsou, E., and Giannakouros, T. (2001) Cloning and characterization
of an alternatively spliced form
of SR protein-specific kinase 1 that interacts specifically with Scaffold Attachment Factor-B. J. Biol. Chem. 276, 40175-40182.
17. Nikolakaki,
E., Du, C., Lai, J., Giannakouros, T., Cantley, L., and Rabinow, L. (2002) Phosphorylation by LAMMER
protein kinases: Determination of aconsensus site, identification of in vitro
substrates and implications for substrate preferences. Biochemistry 41,
2055-2066.
18. Mylonis,
19. Pyrpasopoulou, A., Kotoula, V., Cheva, A., Hytiroglou, P., Nikolakaki, E., Magras, I.N.,
Tsiboukis, T.D. and Karkavelas, G. (2004) Bone morphogenetic protein
expression in newborn rat kidneys after prenatal exposure to radiofrequency
radiation. Bioelectromagnetics
25, 216-227.
20. Kotoula, V., Barbanis, S., Nikolakaki, E., Koufoyannis, D.,
Papadimitriou, C.S. and Karkavelas, G.
(2004) Relative
expression of human telomerase catalytic subunit (hTERT) transcripts in
astrocytic gliomas. Acta Neuropathol. 107, 443–451.
21. Antoniadis, A., Poulios,
22. Nikolakaki,
E., Drosou, V., Sanidas,
23. Karetsou, Z., Emmanouilidou, A., Sanidas,
24. Tsianou, D., Tzitzira, A., Nikolakaki, E., Giannakouros T.,
Bonanou, S. and Georgatsou, E. (2009) The enzymatic activity of SR
protein kinases 1 and 1a is negatively affected by interaction with Scaffold
Attachment Factors B1 and 2.
FEBS J. 276, 5212-5227.
25. Tzimopoulos,
D., Sanidas,
26. Phillipidis, N., Nikolakaki, E.,
Sotiropoulos, S. and
27. Sanidas,
28. Giannakouros,
T., Nikolakaki, E., Mylonis,
I., Georgatsou, E. (2010) SRPKs: A small protein kinase family with a large cellular
presence. FEBS J. Under revision.
29. Peidis, P., Voukkalis,