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PERSONAL

 

Date of Birth:                            March 7, 1959

Marital status:                           Married with two children

Country of citizenchip:   Greece

Languages:                               Greek (mother tongue), English (fluent), French (proficient)

 

 

EDUCATION

 

1976 – 1980:                                       Attended Aristotelian University of Thessaloniki, awarded first degree in Chemistry.

1981 – 1987:                                       Graduate student in the Laboratory of  Biochemistry, Department of Chemistry, Aristotelian University of Thessaloniki (June 1985 - July 1987, Military service).

1987 - 1988:                                        Post-doctoral fellow in the Laboratory of Biochemistry, Department of Chemistry, Aristotelian Univesrity of Thessaloniki.

June 1, 1989 - October 10, 1989:      Short-term EMBO fellow in Dr. Magee's laboratory in the Department of Eukaryotic Molecular Genetics, National Institute for Medical Research, London.

January  1991 - December 1992:      Post-doctoral fellow in Dr. Magee's laboratory, (long-term EMBO fellowship) in the Department of Eukaryotic Molecular Genetics, National Institute for Medical Research, London.

POSITIONS

 

1988 - 1994            Lecturer in the Laboratory of Biochemistry, Department of Chemistry, Aristotelian University of Thessaloniki.

1994 - 2003            Assistant Professor in the Laboratory of Biochemistry, Department of Chemistry, Aristotelian University of Thessaloniki.

2003 - present        Associate Professor in the Laboratory of Biochemistry, Department of Chemistry, Aristotelian University of Thessaloniki.

 

 

AWARDS - FELLOWSHIPS

 

June 1989 - October 1989                 Short-term EMBO fellowship

January 1991 - September 1992       Long-term EMBO fellowship

 

 

TEACHING

 

More than 15 years  of Biochemistry, Control of Metabolism and Molecular Biology courses and laboratories for undergraduate and graduate students of the Chemistry and Pharmaceutical Department of the Aristotelian University of Thessaloniki

 

 

STUDENT THESIS SUPERVISION

 

More than 25 undergraduate, 4 M.Sc and 4 PhD.

 

 

SCIENTIFIC SOCIETIES

 

o American Society for Biochemistry and Molecular Biology

o British Biochemical Society

o Hellenic Society of Biochemistry and Molecular Biology

o Hellenic Chemical Society

 

 

SCIENTIFIC ACTIVITY

 

o 28 publications in peer-reviewed journals

o More than 600 non-self citations, H-index 15, Total impact factor over 110, average 4

o 40 international and national presentations, 5 invited lectures.

FUNDING

 

Coordinator of the following Research Grants

o 1994 -1996 Greek-German bilateral cooperation financed by the General Secretariat for Research & Technology, entitled “Purification, characterization and cloning of the protein kinase that was found to be associated in vivo with the lamin B receptor” (4,000,000 dr).

o 1996 - 1998  Program for scientific research financed by the General Secretariat for Research & Technology (ΠENEΔ), entitled “Modulation of the biological activity of lamin B receptor by a novel serine/threonine protein kinase - Cloning and characterization of this kinase” (8,000,000 dr).

o 1998 - 2000 Program for scientific research financed by the National Health Council (KEΣΥ), entitled “Chromatin condensation during spermatogenesis” (2,000,000 dr).

o 2001 - 2003 Greek-French bilateral cooperation financed by the General Secretariat for Research & Technology, entitled “Characterization of SRPK and LAMMER protein kinases”  (4,200,000 dr).

o 2003 - 2005 Program for basic research financed by the Greek Ministry of Education and the European Community (HPAKΛEITOΣ), entitled “Biological function and protein interactions of SRPK1” (35,000 €).

o 2005 - 2007 Program for basic research financed by the Greek Ministry of Education and the European Community (ΠΥTHAGOPAS II), entitled “Control of differentiation and apoptosis of human erythroleukaemic cells by SRPK1 – Subcellular distibution of the kinase” (40,000 €).

o 2010 - 2012 Greek-French bilateral cooperation financed by the General Secretariat for Research & Technology, entitled “The role of SR protein kinases in the regulation of p53-dependent and independent programmed cell death”  (20,000 €).

 

Participant in the following Research Grants

o 1999 - 2001 Program for scientific research financed by the General Secretariat for Research & Technology (ΠENEΔ), entitled “Self-assembly of the nuclear envelope - Mechanism, regulation and development of inhibitors” (50,000,000 dr).

o 2001 - 2004  Program for scientific research financed by the General Secretariat for Research & Technology (ΠENEΔ), entitled “Heterochromatin protein 1 (HP1) as a molecular marker in cancer” (40,000,000 dr).

o 2000 - 2006 Program for scientific research financed by the General Secretariat for Research & Technology (EΠAN), entitled “Development of stem cell techonolgy in Greece” (300,000 €).

 

 

COLLABORATIONS

 

o Medical School, University of Ioannina (Dr.. S.D. Georgatos)

o Medical School, University of Ioannina (Dr.. T. Papamarcaki)

o Medical School, University of Thessaly (Dr. E. Georgatsou)

o Université Paris 11, CNRS Orsay, France (Dr. L. Rabinow)

o Department of Pathology, University of Tennessee Health Science Center, USA. (Dr. R.E. Scott)

o Dana Farber Cancer Institute, Harvard Medical School, Boston, USA (Dr. L.C. Cantley).

o Department of Pharmacology, School of Medicine, University of California-Irvine, USA (Dr. P. Sassone-Corsi).

o Applied Biosystems, Darmstadt, Germany (Dr. V. Kruft)

o Laboratory of Statistical Genetics, The Rockefeller University, New York, USA (Dr. L. Iakoucheva)

o Department of Physiology, Biophysics and Neuroscience, Center for Research and Advanced Studies, México (Dr. L. González-Mariscal).

 

 

CURRENT RESEARCH INTERESTS

 

o Post-translational modifications of proteins

o Protein phosphorylation and signal transduction

o Biochemical properties, function and regulation of protein kinases phosphorylating Serine/Arginine(SR) motifs

o Attachment of chromatin to the innner nuclear membrane in somatic and germ cells

o Involvement of nuclear matrix in the regulation of gene expression

 

 

SCIENTIFIC REVIEWER  

 

o Molecular and Cellular Biology

o Oncogene

o BMC Biochemistry

o Acta Biochimica et Biophysica Sinica

 

 

PUBLICATIONS  (in peer reviewed journals)

 

1.     Giannakouros, T. and Georgatsos J.G. (1988) A high yield cell-free system of protein-synthesis from mouse liver. Int. J. Biochem.  20,  511-519.

 

2.     Simos, G., Giannakouros, T. and Georgatsos, J.G. (1989) Plant β-galactosidases: Purification by affinity chromatography and properties. Phytochemistry 28, 2587-2592.

 

3.     Giannakouros, T., Nikolakaki, E. and Georgatsos, J.G. (1990) Concentration dependent effects of natural polyamines on peptide chain initiation and elongation in a cell-free system of protein synthesis. Mol. Cell. Biochem. 99, 9-19.

 

4.     Nikolakaki, E., Giannakouros, T.  and Georgatsos, J.G. (1990) Inhibition of protein synthesis by acetyl-coenzyme A in a cell-free system. Possible involvment of protein acetylation in the regulation of regulation of translation. BioFactors 2, 255-258.

 

5.     Giannakouros, T., Karagiorgos, A. and Simos, G. (1991) Expression of β-galactosidase multiple forms during barley (Hordeum vulgare) seed germination. Separation and characterization of enzyme isoforms.  Physiol. Plant. 82, 413-418.

 

6.     Magee, A.I., Newman., C.M., Giannakouros, T., Hancock, J.F., Fawell, E. and Armstrong, J. (1992)  Lipid modifications and function of the ras superfamily of proteins. Biochem. Soc. Trans. 20, 497-499.

 

7.     Giannakouros, T., Armstrong, J. and Magee, A.I. (1992)  Protein prenylation in Schizosaccharomyces pombe. FEBS  Lett. 297, 103-106.

 

8.     Newman, C.M., Giannakouros, T., Hancock, J.F., Fawell, E.H., Armstrong, J. and Magee, A.I. (1992) Post-translational properties of Schizosaccharomyces pombe YPT proteins. J. Biol. Chem. 267, 11329-11336.

 

9.     Giannakouros, T., Newman, C.M., Armstrong, J. and Magee, A.I. (1993) Processing of the small GTP-binding protein SpYPT1 in Schizosaccharomyces pombe  and in mammalian COS cells. Biochem. Biophys. Res. Commun. 192, 983-990.

 

10.   Giannakouros, T., Newman, C.M., Craighead, M.W., Armstrong, J. and Magee, A.I. (1993)  Post-translational processing of Schizosaccharomyces pombe  YPT5 protein. J. Biol. Chem. 268, 24467-24474.

 

11.   Nikolakaki, E., Simos, G., Georgatos, S.D. and Giannakouros, T. (1996) A nuclear envelope bound kinase phosphorylates arginine-serine motifs and modulates interactions between the lamin B receptor and other nuclear proteins. J. Biol. Chem. 271, 8365-8372.

 

12.   Nikolakaki, E., Meier, J., Simos, G., Georgatos, S.D. and Giannakouros, T. (1997) Mitotic phosphorylation of the lamin B receptor (LBR) by a serine/arginine kinase and p34cdc2 J. Biol. Chem. 272,  6208-6213.

 

13.   Papoutsopoulou, S., Nikolakaki, E., and Giannakouros, T. (1999) SRPK1 and LBR protein kinases show identical substrate specificities. Biochem. Biophys. Res. Commun. 255, 602-607.

 

14.   Papoutsopoulou, S., Nikolakaki, E., Chalepakis, G., Kruft, V., Chevaillier, P., and Giannakouros, T. (1999)  SR protein-specific kinase 1 is highly expressed in testis and phosphorylates protamine 1. Nucleic Acids Res.  27,  2972-2980.

 

15.   Nikolakaki, E., Fissentzidis, A., Giannakouros, T., and Georgatsos, J.G. (1999) Purification and characterization of a dimer form of the cAMP-dependent protein kinase of mouse liver cytosol.  Mol. Cell. Biochem. 197, 117-128.

 

16.   Hartmann, A., Rujescu, D., Giannakouros, T., Nikolakaki, E., Goedert, M., Mandelkow,  E., Gao, Q.S., Andreadis, A., and Stamm, S. (2001) Regulation of alternative splicing of human tau exon 10 by phosphorylation of splicing factors. Mol. Cell. Neurosci. 18, 80-90.

 

17.   Polioudaki, H., Kourmouli, N., Drosou, V., Bakou, A., Theodoropoulos P.A., Singh, P.B., Giannakouros, T., and Georgatos S.D. (2001) Histones H3/H4 form a tight complex with the inner nuclear membrane protein LBR and Heterochromatin Protein 1. EMBO  Rep.  2, 920-925.

 

18.   Nikolakaki, E., Kohen, R., Hartmann, A.M., Stamm, S., Georgatsou, E., and Giannakouros, T. (2001) Cloning and characterization of an alternatively spliced form of SR protein-specific kinase 1 that interacts specifically with  Scaffold Attachment Factor-B. J. Biol. Chem.  276, 40175-40182.

 

19.   Nikolakaki, E., Du, C., Lai, J., Giannakouros, T., Cantley, L., and Rabinow, L. (2002) Phosphorylation by LAMMER protein kinases: Determination of a consensus site, identification of in vitro substrates and implications for substrate   preferences. Biochemistry 41, 2055-2066.

 

20.   Mylonis, I. and  Giannakouros, T. (2003) Casein kinase II phosphorylates and activates the SR protein-specific kinase 1. Biochem. Biophys. Res. Commun. 301, 650-656.

 

21.   Scott, R.E., Giannakouros, T., Gao, S., and Peidis, P. (2003) Functional potential of P2P-R: A role in the cell cycle and cell differentiation related to its interactions with proteins that bind to matrix associated regions of DNA? J. Cell. Biochem. 90, 6-12.

 

22.   Mylonis, I., Drosou, V., Brancorsini, S., Nikolakaki, E., Sassone-Corsi, P. and Giannakouros, T. (2004) Temporal association of protamine 1 with the inner nuclear membrane protein LBR during spermiogenesis. J. Biol. Chem. 279, 11626-11631.

 

23.   Nikolakaki, E., Drosou, V., Sanidas, I., Peidis P., Papamarcaki, T., Iakoucheva, L.M. and Giannakouros, T. (2008) RNA association or phosphorylation of the RS domain prevents aggregation of  RS domain-containing proteins. Biochim. Biophys. Acta 1780, 214-225.

 

24.   Meklat, F., Zhang, Y., Shahriar, M., Ahmed, S.U., Li, W., Voukkalis, N., Wang, Z., Zhang, J., Mastulov, S., Jewell, A., Giannakouros, T. and  Lim, S.H. (2009) Identification of protamine 1 as a novel cancer-testis antigen in early chronic lymphocytic leukaemia. Br. J. Haematol. 44, 660-666.

 

25.   Ahmed, S.U., Meklat, F., Shahriar, M., Zhang, J., Mastulov, S., Giannakouros, T., Jewell, A., Zhang, Y. and Lim, S.H. (2009) SEMG-1 expression in early stage chronic lymphocytic leukemia. Cytotherapy 11, 238-244.

 

26.   Tsianou, D., Nikolakaki, E., Tzitzira, A., Bonanou, S., Giannakouros T. and  Georgatsou, E. (2009) The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with Scaffold Attachment Factors B1 and 2. FEBS J. 276, 5212-5227.

 

27.   Peidis, P., Giannakouros T., Burow, M.E., Williams, R.W and Scott, R.E. (2010) Systems genetics analyses predict a transcription role for P2P-R: Molecular confirmation that P2P-R is a transcriptional co-repressor. BMC Systems Biology Feb 25; 4:14.

 

28.   Giannakouros, T., Nikolakaki, E., Mylonis, I., Georgatsou, E.  (2010) SRPKs: A small protein kinase family with a large cellular presence. FEBS J. Under revision.

 

29.     Peidis, P., Voukkalis, N. Aggelidou, E., Georgatsou, E., Hadzopoulou-Cladaras, M., Scott, R.E., Nikolakaki, E. and Giannakouros T. (2010) SAFB1 interacts with and suppresses the transcriptional activity of p53. FEBS Lett. Submitted for publication.

 

 

PUBLICATIONS  (in books)

 

1.     Hancock, J., Cadwallader, K., Magee, A.I., Newman, C., Giannakouros, T., Fawell, E., Armstrong, J., Paterson, H. and Marshall, C. (1991)  Prenylation of ras and ras-related proteins. In The Superfamily of ras related genes, D. Spandidos (ed.) Plenum Press, N.Y., U.S.A. 15-22.

 

2.     Magee, A.I., Hancock, J.F., Fawell, E., Giannakouros T., Newman, C.M., Marshall, C. and Armstrong, J. (1992) The ras superfamily: Post-translational modifications and functional regulation. In Growth  factors of the vascular and nervous systems, C. Lenfant,  R. Paoletti, A. Albertini (eds) Basel, Karger 1-5.

 

  3.   Giannakouros, T. and Magee, A.I. (1992)  Protein prenylation and associated modifications. In Lipid modifications of proteins   M.J. Schlesinger (ed.) CRC Press Inc., Boca Raton, Florida, U.S.A. 133-160.

 

4.     Newman C.M.H., Giannakouros, T. and Magee, A.I. (1993) Mutation of a putative phosphorylation site of a yeast YPT protein using the Sculptor in vitro mutagenesis kit. Life Science News (Pharmacia) 12, 7-8.

 

5.     Giannakouros, T. (1997)   Lipid modifications of proteins. In Protein structure analysis. Preparation, characterization and microsequencing R.M. Kamp, T. Choli-Papadopoulou and B. Wittmann-Liebold (eds) Springer-Verlag, Berlin Heidelberg 269-276.