CURRICULUM VITAE


Cynthia H. Panagiotidis
Name:  Cynthia H. Panagiotidis
Title:  Independent Biosciences Consultant
Date of birth: 1958
Place of birth:  Vermont, U.S.A.
Citizenship:  USA & Greece (naturalized)
Address:
    Ikismos Agias Anastasias
   57006 Vasilika, Greece
          Tel.:  +30 2396 031368
          E-mail:  cpanagi@pharm.auth.gr
          Web page:  http://users.auth.gr/cpanagi
    home:  Ikismos Agias Anastasias
   57006 Vasilika, Greece
          Tel.:  +30 2396 031368

EDUCATIONAL BACKGROUND

   1980 B.A. Chemistry & Biology (double major) Bryn Mawr College, Bryn Mawr, PA (US)
   1983 M.Phil. Pharmacology YALE University, New Haven, CT (US)
   1989 Ph.D. Pharmacology YALE University, New Haven, CT (US)


POSITIONS HELD

Self-employed Independent Biosciences Consultant July 2012 to present
School of Pharmacy Senior Research Scientist January 2000 to
   Aristotle University      
   54124 Thessaloniki, Greece      
Department of Microbiology Associate Research Scientist April 1994 to June 1998
   Columbia University Postdoctoral Research Fellow July 1990 to March 1994
   College of Physicians & Surgeons      
   New York, NY 10032      
Department of Pharmacology Postdoctoral Research Scientist September 1989 to June 1990
   Yale University      
   School of Medicine      
   New Haven, CT      
Department of Chemistry Research Assistant August 1980 to August 1981
   Haverford College      
   54124 Haverford, PA      
Department of Gastroenterology Research Assistant June 1979 to August 1979
   Merck Co.      
   West Point, PA      

HONORS & PROFESSIONAL SOCIETIES

   Cystic Fibrosis Foundation Fellow 1991-1993
   National Cancer Institute Fellow 1993-1994
   American Academy for the Advancement of Science from 1990
   American Society for Microbiology from 1990
   Hellenic Society for Biochemistry & Molecular Biology from 2009


RESEARCH INTERESTS

• Molecular Biology of Neurodegenerative Diseases (Alzheimer’s Disease, Parkinsons Disease, CJD, ALS, etc)

• Development of biomarkers for disease diagnosis and for evaluation of disease stage
• Molecular basis of the genetic susceptibility and resistance to disease in humans and livestock animals.
• Molecular mechanisms of infectious disease transmission
• Development of pharmaceuticals and immunotherapies for the treatment and prevention of neurodegenerative diseases
• Pharmaceutical manipulation of neurotransmitter reuptake systems

RESEARCH FUNDING

• Cystic Fibrosis Foundation Fellow 1991-1993

• National Cancer Institute Fellow 1993-1994

• FAIR SC. Surveillance and Diagnosis of Ruminant TSE PL 98-7021, European Commission, Agriculture Directorate General 1998-2001 (project participant).

• BIOMED 2 SC. Prion diseases: mechanisms of transmission and identification of target for potential therapeutics. PL CT 98-6040, European Commission, 1998-2001 (project participant).

• BMH4-CT98-6040. Prion Diseases: Mechanism of transmission and identification of targets for potential therapeutics. European Commission, Research Directorate General, 1998-2001 (project participant).

• Peptide identification with potential diagnostic and therapeutic use of neurodegenerative disorders, 97 EKBAN2-1.2-35, Greek Ministry of Development, 1998-2001 (project participant).

• FAIRJ-CT98-7021. The establishment of a European network for the surveillance of ruminant TSE and the standardization and harmonization of the process and criteria for the identification of suspected cases. European Commission, Agriculture Directorate, 1999-2002 (project participant).

• Detection and characterization of Creutzfeldt-Jacob disease in Greece. Greek Center for Control of Special Diseases, 2001-2005 (project participant).

• QLK5-2002-8066.”TSE and Fish:”Evaluation of the possible transmission of prions (Scrapie and BSE) to different fish species. European Commission, 2002-2007 (project participant).

• QLK5-2002-0959. “SRTSENETWORK:” European Network of Surveillance and Control of TSE in Small Ruminants (with emphasis on epidemiology, pathology and diagnostic tests). European Commission, 2003-2006 (project participant).

• FOOD-CT-2004-506579. “NEUROPRION:”Prevention, Control, Treatment, Management and Risk Analysis of Prion Diseases. European Commission, 2003-2008 (project participant).

• Distribution of endogenous PrPC and evaluation of PrPSc uptake in the intestines of Sparus auratus orally challenged with natural scrapie.Greece-Italy Joint Project: ÅÐAv.M.4.3.6.1.å, Greek General Secretariat for Research and Technology, 2006-2008 (project participant).

• FOOD-CT-2006-036353. “GoatBSE:”, Proposal for improvement of goat TSE discriminative diagnosis and susceptibility based assessment of BSE infectivity in goat milk and meat . European Commission, 2006-2011 (project participant).

 

PUBLICATIONS

1. Lazo, J.S. and C.J. Humphreys. Lack of metabolism as the biochemical basis of bleomycin-induced pulmonary toxicity. Proc. Natl. Acad. Sci. USA 80: 3064-3068, 1983.

2.      Lin, T.-S., R.-X. Zhou, K.J. Scanlon, W.F. Brubaker, Jr., J.J.S. Lee, K. Woods, C. Humphreys and W.H. Prusoff.  Synthesis and biological activity of several amino nucleosie-platinum (II) complexes.  J. Med. Chem.  29: 681-686 1986.

3.      Humphreys, C.J., J. Levin and G. Rudnick.  Antidepressant binding to porcine and human platelet serotonin transporters. Mol. Pharmacol.  33:  657-663, 1988.

4.      Humphreys, C.J., D. Cassel and G. Rudnick.  2-Iodoimpramine, a novel ligand for the serotonin transporter. Mol. Pharmacol.  36: 620-626, 1989.

5.      Humphreys, C.J. and G. Rudnick. Substrate and inhibitor binding and translocation by the platelet plasma membrane serotonin transporter. Biochem. Soc. Trans.  19: 95-98,1991.

6.      Rudnick, G and C.J. Humphreys.  Platelet serotonin transporter.  Meth. Enzymol. 215: 213-224, 1992.

7.      Panagiotidis, C.H., M. Reyes, A. Sievertsen, W. Boos and H.A. Shuman. Characterization of the structural requirements for asembly and nucleotide binding of an ATP-binding cassette transporter. J. Biol. Chem.  268: 23685-23696, 1993.

8.      Shuman, H.A. and C.H. Panagiotidis. Tinkering with transporters: periplasmic  binding protein-dependent maltose transport in E. coli. J. Bioenerg. Biomemb.  25: 613-620,1993.

9.      Humphreys, C.J., S.C. Wall and G. Rudnick.  Ligand binding to the serotonin transporter: Equilibria, kinetics and ion dependence.  Biochemistry  33: 9118-9125, 1994.

10.    Covitz, K.-M. Y., C.H. Panagiotidis, L.-I. Hor, M. Reyes, N.A. Treptow and H.A. Shuman. Mutations that alter the transmembrane signalling pathway in an ATP-binding cassette (ABC)-transporter.  EMBO J.  13: 1752-1759, 1994.

11.    Panagiotidis, C.H. and H.A. Shuman.  Maltose transport in E. coli:  Mutations that uncouple ATP hydrolysis from transport.  Meth. Enzymol. 292:30-39, 1998.

12.    Panagiotidis, C.H., W. Boos and H.A. Shuman.  The ATP-binding cassette subunit of the maltose transporter MalK antagonizes MalT, the activator of the Escherichia coli mal regulon. Mol. Microbiol., 30: 535-546, 1998.

13.    Reich-Slotky, R., C. Panagiotidis, M. Reyes and H.A. Shuman.   The Detergent-Soluble Maltose Transporter Is Activated by Maltose Binding Protein and Verapamil. J. Bacteriol. 182: 993-1000, 2000.

14.    Billinis C., Panagiotidis, C.H., Psychas, V., Argyroudis, S., Nicolaou, A., Leontides, S., Papadopoulos, O. and Sklaviadis, T.  Prion protein gene polymorphisms in natural goat scrapie. J. Gen. Virol., 83:713-721, 2002.

15.    Sachsamanoglou, M., Paspaltsis, I., Petrakis, S., Verghese-Nikolakaki, S., Panagiotidis, C.H., Voigtlander, T., Budka, H., Langeveld, J.P. and Sklaviadis, T. Antigenic profile of human recombinant PrP: generation and characterization of a versatile polyclonal antiserum. J. Neuroimmunol., 146: 22-32, 2004.

16.    Bloukas, G., Kotta, K., Kunze, S., Gnoth, S., Metze, J., Bodemer, W., Panagiotidis, C.H., Sklaviadis, T. Application of the Atomic Force Microscopy Technique to the Study of Recombinant Prion Protein and its Potential Ligand Interactions. Rev. Clin. Pharmacol. Pharmacokin., Intern. Edition 18: 45-48, 2004.

17.    Lioliou, E.E., Mimitou, E.P., Grigoroudis, A.I., Panagiotidis, C.H., Panagiotidis, C.A. & Kyriakidis, D.A. Phosphorylation activity of the response regulator of the two-component signal transduction system AtoS–AtoC in E. coli. Biochim. Biophys. Acta 1725, 257-268, 2005.

18.    Rivera-Milla, E., Oidtmann, B., Panagiotidis C.H., Baier, M., Sklaviadis, T., Hoffmann, R., Zhou, Y., Solis, G.P., Stuermer, C.A., Malaga-Trillo, E. Disparate evolution of prion protein domains and the distinct origin of Doppel- and prion-related loci revealed by fish-to-mammal comparisons. FASEB J. 20, 317-319, 2006.

19.    Theodorou, M.C., Panagiotidis, C.A., Panagiotidis, C.H., Pantazaki, A.A. & Kyriakidis, D.A.  Involvement of the AtoS-AtoC signal transduction system in poly-(R)-3-hydroxybutyrate biosynthesis in Escherichia coli. Biochim. Biophys. Acta  1760: 896-906, 2006.

20.    Ekateriniadou, L.V., Panagiotidis, C.H., Terzis, A., Ploumi, K., Triantafyllidis, A., Deligiannidis, P., Triantaphyllidis, C. & Sklaviadis, T.  Genotyping for PrP gene polymorphisms in rare Greek breeds of sheep. Vet. Rec. 160: 194-195, 2007.

21.    Favre-Krey, L., Theodoridou, M., Boukouvala, E., Panagiotidis, C.H., Papadopoulos, A.I., Sklaviadis, T. & Krey, G. Molecular characterization of a cDNA from the gilthead sea bream (Sparus aurata) encoding a fish prion protein. Comp Biochem Physiol B Biochem Mol Biol. 147: 566-573, 2007.

22. Matta, M.K., Lioliou, E.E., Panagiotidis, C.H., Kyriakidis, D.A. & Panagiotidis, C.A Interactions of the antizyme AtoC with regulatory elements of the Escherichia coli atoDAEB operon. J. Bacteriol. 189: 6324-6332, 2007.

24.  Ekateriniadou, L.V., Kanata, E., Panagiotidis, C., Nikolaou, A., Koutsoukou, E., Lymberopoulos, A.G., Sklaviadis, T. PrP genotypes in scrapie-affected sheep in Greece-The contribution of the AHQ 1 polymorphism.  Small Rumin. Res. 73: 142-149, 2007.

24. Petrakis, S., Irinipoulou, T., Panagiotidis, C.H., Engelstein, R., Lindstrom, J., Orr-Urtreger, A., Gabizon, R., Grigoriadis, N. & Sklaviadis, T. Cellular prion protein co-localizes with nAChR beta4 subunit in brain and gastrointestinal tract. Eur. J. Neurosci. 27: 612-620, 2008.

25.  Kunze, S., Lemke, K., Metze, J., Bloukas, G., Kotta, K., Panagiotidis, C.H., Sklaviadis, T. & Bodemer, W. Atomic force microscopy to characterize the molecular size of prion protein. J Microsc. 230: 224-232, 2008.

26.    Arrizubieta, M.J., Kanata. E., Keklikoglou, I., Papasavva-Stylianou, P., Toumazos, P., Panagiotidis, C.H., Sklaviadis, T. Design and validation of a high-throughput assay to detect codon 146 polymorphisms in the caprine prion protein gene. Anal. Biochem. 393:229-233, 2009.

27.    Vaccari, G.*, Panagiotidis, C.H.*, Acin, C., Peletto, S., Barillet, F., Acutis, P., Bossers, A., Langeveld, J., Van Keulen, L., Sklaviadis, T., Badiola, J.J., Andreéoletti, O., Groschup, M.H., Agrimi, U., Foster, J. & Goldmann, W. State-of-the-art review of goat TSE in the European Union, with special emphasis on PRNP genetics and epidemiology. Vet. Res. 40(5):48, 2009.

28.    Salta, E.*, Panagiotidis, C.*, Teliousis, K., Petrakis, S., Eleftheriadis, E., Arapoglou, F., Grigoriadis, N., Nicolaou, A., Kaldrymidou, E., Krey, G. & Sklaviadis, T. Evaluation of the possible transmission of BSE and scrapie to gilthead sea bream (Sparus aurata). PLoS One.  4(7):e6175, 2009.

29.    Málaga-Trillo, E., Salta, E., Figueras, A., Panagiotidis, C., Sklaviadis, T. Fish models in prion biology: underwater issues. Biochim. Biophys. Acta 1812:402-414, 2011.

30.   Goldmann, W., Acin, C., Acutis, P., Agrimi, U., Andreoletti, O., Barillet, F., Panagiotidis, C., Peletto, S., Sklaviadis, T., Vaccari, G., Langeveld, J., Bossers, A. Survey of caprine PRNP and SPRN allele frequencies in the European Union and their relevance for potential TSE control by selective breeding strategies in goats. Prion 7S:26, 2013.

31.    Niedermeyer, S., Eiden, M., Toumazos, P., Papasavva-Stylianou, P., Sklaviadis, T., Panagiotidis, C., Langeveld, J., Bossers, A., Kuczius, T., Kaatz, M., Groschup, M.H., Fast, C. Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus. Vet. Res. 47:99, 2016.

 

 

PATENT

International Patent: WO/2004/037854

Title: PRION PROTEIN-BINDING PEPTIDE SEQUENCES

Inventors: Sklaviadis, Theodoros, Panagiotidis, Cindy,  Paspaltsis, John, Karageorgis,Basil

Publication date: 6-May-2004

 

CONGRESS PROCEEDINGS

1.      Rudnick, G., C.J. Humphreys and G.E. Dean (1985).  Serotonin transport by platelet plasma and granule membranes.  Annals of the New York Academy of Sciences 456: p.277-278, New York, N.Y.

2.      Panagiotidis, C.H. and H. Shuman (1991).  The role of MalFGK complex in active transport of maltose.  The Maltose System: Molecular genetics and cell biology.  Kostanz, Germany.

3.      Panagiotidis, C.H., M. Reyes, A. Sievertsen and H. Shuman (1991).  Specific sites required for binding of purine nucleotides to a procaryotic homolog of CFTR.  5th North American Cystic Fibrosis Conference, Dallas, Texas.

4.      Panagiotidis, C.H. (1992).  Protein Interactions during maltose active transport.  92nd General Meeting of the American Society for Microbiology, New Orleans, LA.

5.      Panagiotidis, C.H., W. Boos and H. Shuman (1995).  Signalling and transcriptional control by an ABC transporter.  50th Meeting on Molecular Genetics of Bacteria and Phages, Cold Spring Harbor, N.Y.

6.      Panagiotidis, C. H. (1996).  The direct role of an ABC transporter in transcription.  Snowbird Analytical Genetics Meeting, University of Utah, Salt Lake City, Utah.

7.      Lioliou, E.E., Panagiotidis, H.C., Typas, M.A., Panagiotidis, C.A. & Kyriakidis, A.D. (2000) Polyamine Biosynthesis and Metabolic Control of a Two-Component signal transduction system.  2nd International Conference of the Chemical Societies of the South-Eastern European Countries on Chemical Sciences for Sustainable Development, Halkidiki, Greece, Book of Abstracts Vol. I: p.164

8.      Lioliou, E.E., Panagiotidis, C.H., Typas, A.M., Panagiotidis, C.A., & Kyriakidis, D. (2000) Studies on a Two-Component Signal Transduction System, AtoS-AtoC, in E. coli. Proceedings of the 52nd Meeting of the Hellenic Society of Biochemistry and Molecular Biology, HSBMB Newsletter, Vol. 47: pp. 61-65.

9.      Lioliou, E.E., Panagiotidis, C.H., Panagiotidis, C.A. & Kyriakidis, D.A. (2001) Mapping of the Antizyme Responsive Element on E. coli Chromosome. Proceedings of the 53rd Meeting of the Hellenic Society of Biochemistry and Molecular Biology, HSBMB Newsletter, Vol. 48: pp. 223-226.

10.     Kotta, K., Paspaltsis, I., Panagiotidis, C. H., Sklaviadis, T (2002). In vitro conversion of sheep recombinant PrPC to PrPSc, International Conference for Transmissible Spongiform Encephalopathies, “New perspectives for Prion Therapeutics”, Paris (poster)p. 17.

11.     Paspaltsis, M. Geissen, J. Langeveld, A. Oelschlegel, M. H. Groschup, C. Panagiotidis and T. Sklaviadis (May 2004).  Characterization of prion protein binding peptides.  Prion 2004 First International Conference of the European Network of Excellence Neuroprion, Paris (poster), p.155.

12.  S. Kunze, K. Lemke, J. Metze, G. Bloukas, K. Kotta, C.H. Panagiotidis, T. Sklaviadis, W. Bodemer (2005) Αtomic Force Microscopy to investigate heavy metal ions as modulator of prion protein binding. Prion 2005 – Between fundamentals and society’s needs, Düsseldorf (poster STRCT-06) p. 335.

13.     Panagiotidis C., Elefteriadis, E., Arapoglou, F., Teliousis, K., Kaldrymidou, E., Krey, G. and Sklaviadis, T. (2006)  Evaluation of the possible transmission of prions to sea bream. 31st FEBS Congress, Istanbul, Turkey, 24-29 June 2006. The FEBS Journal 273: Suppl. 1, PP621, p. 244.

14.   Salta E.,Panagiotidis C.,Petrakis S.,Elefteriadis E.,Arapoglou F.,Karagiannis D.,Aggelidis P.,Teliousis K.,Kaldrymidou E.,Krey G.,Sklaviadis T.  (2007) Evaluation of Possible Transmission of Prions to Sea Bass. Prion 2007, Edinburgh (poster 3.29), p.54.

15.   Salta E.,Panagiotidis C.,Petrakis S.,Elefteriadis E., Karagiannis D.,Aggelidis P.,Teliousis K.,Kaldrymidou E.,Krey G.,Sklaviadis T.  (2008) Evaluation of the possible transmission of prions to the two closely related teleost species, sea bream and sea bass. Prion 2008, Madrid (poster 3.18), p.70.

16.     Peletto, S., Acin, C., Vaccari, G., Panagiotidis, C.H., Chiappini, B., Badiola, J.J., Sklaviadis, T., Bossers, Goldmann, W., Acutis, P.L. (2009) Molecular characterization of the putativeregulatory regions of the prion protein gene (PRNP) in goats. Prion2009, Halkidiki, Greece (poster P7.8), p.156.

17.  Fast, C., Niedermeyer, S., Eiden, M., Toumazos, P., Papasavva-Stylianou, P., Ioannou, I., Sklaviadis, T., Panagiotidis, C., Bossers, A., Langeveld, J., Nonno, R., Kuczius, T., Kaatz, M., Groschup, M.H., (2014) Characterization of clinically suspect goat TSE cases from Cyprus. Prion 8:68, 2014.

18. Panagiotidis, C.H., Stoikou M. & Panagiotidis C.A. (2012) Modulation of a viral E3 ubiquitin ligase activity by its physical interaction with a chromatin architectural protein. Proceedings of the 63rd Meeting of the Hellenic Society of Biochemistry and Molecular Biology, HSBMB Newsletter, Vol. 58, 01, p. 46, 9-11 November 2012, Heraklion, Crete, Greece.

19. Kalfopoulou, E.A., Chatsisvili, A., Panagiotidis, C.H. and Panagiotidis, C.A. (2014) A study on the effects of chaperone fusions on protein folding and activity using firefly luciferase fusions with DnaK and GroEL. FEBS – EMBO 2014 Conference, August 28-Setember 4, 20014, Paris, France, Abstract 3684.