CURRICULUM VITAE
Zotou Anastasia
Professor
Laboratory of Analytical Chemistry
Department of Chemistry
Aristotle University of
Thessaloniki
54124
Tel.: +30 2310 997746
E-mail:azotou@chem.auth.gr
Education
Postdoctoral
studies: School of Pharmacy and Pharmacology, Laboratory of
Pharmaceutical Analysis, University of Bath, England (12 months, 1991-92).
Ph.D.: Analytical Chemistry,
Department of Chemistry, Aristotle University of Thessaloniki (1989).
Supplementary degree in Applied Environmental Chemistry: University of Umeå, Sweden (1980).
Degree: Philosophie Candidatexamen (equivalent to B.Sc.), University of Umeå, Sweden (1979), Major: Chemistry.
Academic
status
Full Professor, February 2014-present
Department of Chemistry
Associate Professor, 2004-2014
Department of Chemistry
Assistant Professor, 1995-2004
Department of Chemistry
Lecturer, 1990-1995
Department of Chemistry
Professional
Association Memberships
Member of Greek Chemists’ Association.
Member of British Chromatographic Society
(1992-1995).
Member of Greek Toxicology Society.
Teaching
Undergraduate level:
• Basic Principles of Analytical Chemistry, Department of Chemistry, AUTh
• Instrumental
Chemical Analysis I, Department of Chemistry, AUTh
• Special
Methods of Analysis, Department of Chemistry, AUTh
• Bioanalytical
Chemistry, Department of Chemistry, AUTh
• Analytical
Chemistry, Department of Geology, AUTh
• Analytical
Chemistry, Department of Agriculture, AUTh
Postgraduate level: "Quality Control-Chemical Analysis-Environmental Analysis " Department
of Chemistry, AUTh
• Separation Techniques in Chemical
Analysis
• Bioanalytical Methods
• Research and Laboratory Methodology in
Bioanalysis
• Standard Separation Techniques in
Chemical Analysis
• Research and Laboratory Methodology in Quality
Control
Supervision
Supervision of 4 Ph.D. students, 13 postgraduate
students and 18 undergraduate students.
Current Research interests
Separation methods of
analysis:
i) Reversed-Phase High Pressure and/or
Ultra-High Pressure Liquid Chromatography with optical detectors and LC-MS/MS
or HILIC-MS/MS.
ii) Capillary Electrophoresis with optical detection.
iii) Gas Chromatography-Mass Spectrometry. Purge-and-Trap extraction of
volatile compounds.
Coupling
of automated flow methods of analysis with separation techniques: Automated sample preparation by Sequential Injection Analysis coupled
to Liquid Chromatography (SIA-HPLC).
Development and validation of novel
analytical methods for the determination of various analytes such as amines, amino
acids, organic acids, flavonoids, thiols, vitamins, drugs, e.t.c
in various samples (foods and beverages, biological fluids, pharmaceuticals, e.t.c) using the above techniques.
Development
and optimization of sample preparation methods:
Solid Phase Extraction (SPE), Matrix Solis Phase Dispersion (MSPD),
Liquid-Liquid Microextraction etc.
i) Ph.D. Dissertation
“Study of the catalytic
determination of trace amounts of iron(III) and vanadium(V)”,
ii)
Publications in refereed
journals with impact factor and in books
1. “Heavy Metals in Marine Organisms of the North
G. Vasilikiotis, K. Fytianos, A. Zotou
Chemosphere, 12(1), 75-81 (1983).
2. “Indicator Reaction for the Kinetic-Spectrophotometric Determination of
Nanogram Amounts of Iron”
Anastasia
Ch. Zotou and Constantine G. Papadopoulos
Analyst,
112, 787-789 (1987).
3. “Kinetic-Spectrophotometric Determination of Trace Amounts of
Vanadium(V)”
Anastasia
Ch. Zotou and Constantine G. Papadopoulos
Analyst, 115, 323-327 (1990).
4. “Rapid Assay for the Determination of Tolfenamic
Acid in Pharmaceutical Preparations and
Biological Fluids by High-Performance Liquid Chromatography”
É. Papadoyannis, M. Georgarakis, V. Samanidou and A. Zotou
J. Liq. Chromatogr.,
14(15), 2951-2967 (1991).
5. “Kinetic-Spectrophotometric Determination of Molybdenum(VI) and Tungsten(VI)
in Mixtures”
Constantine G. Papadopoulos and Anastasia C. Zotou*
Mikrochim. Acta, 106, 203-210 (1992).
6. “Simultaneous Reversed-Phase Gradient-HPLC Analysis of Anthranilic Acid
Derivatives in Anti-Inflammatory Drugs and Samples of Biological Interest”
I.N. Papadoyannis, A.C. Zotou and V.F.
Samanidou
J. Liq. Chromatogr., 15(11), 1923-1945 (1992).
7. “Determination of Anatoxin-a and Homoanatoxin
in Blue-Green Algal Extracts by High-Performance Liquid Chromatography and Gas Chromatography-Mass Spectrometry”
Anastasia
Zotou, Terry M. Jefferies, Paul A. Brough and Timothy
Gallagher
Analyst,
118, 753-758 (1993).
8. “Comparative Study of Different Solid-Phase Extraction Cartridges in the
Simultaneous RP-HPLC Analysis of Morphine and Codeine in Biological Fluids”
I. Papadoyannis, A. Zotou,
V. Samanidou, G. Theodoridis
and F. Zougrou
J.
Liq. Chromatogr., 16(14), 3017-3040 (1993).
9. “Comparative Study of Solid-Phase Extraction Cartridges in the
Simultaneous Reversed-Phase HPLC Analysis of Bamifylline
and its Major Metabolite AC-
I. Papadoyannis, V. Samanidou, A. Zotou and G. Tsioni
J. Liq. Chromatogr., 16(17), 3827-3845 (1993).
10. “Simultaneous Determination of Bamifylline and
its Major Metabolite AC-119
by RP- HPLC”
I. Papadoyannis, V. Samanidou and A.
Zotou
J. Liq. Chromatogr.,
16(17), 3847-3861 (1993).
11. “Determination of Anatoxin-a, Homoanatoxin and Propylanatoxin in
Cyanobacterial Extracts by HPLC, GC-Mass
Spectrometry and Capillary Electrophoresis”
Terry M. Jefferies, Gavin Brammer, Anastasia Zotou, Paul A. Brough and Timothy
Gallagher
In
"Detection Methods for Cyanobacterial Toxins", The Royal
Society of Chemistry,
1994,
pp. 34-39.
12. “Solid-Phase Extraction and RP-HPLC Analysis of Atropine Sulphate
and Scopolamine-N-Butylbromide
in Pharmaceutical Preparations and Biological Fluids”
I. Papadoyannis, A. Zotou, V. Samanidou and M. Georgarakis
Instrum. Sci. Technol., 22(1), 83-103 (1994).
13. “Highly Selective Simultaneous Determination of eight Inorganic Anions
in Drinking Water by Single Column High Pressure Anion Chromatography”
I. Papadoyannis, V. Samanidou and A.
Zotou
J. Liq. Chromatogr., 18(7), 1383-1403 (1995).
14. “Solid-Phase Extraction Study and RP-HPLC Analysis of Lamotrigine in
Human Biological Fluids and in Antiepileptic Tablet Formulations”
I.N. Papadoyannis, A.C. Zotou and V.F.
Samanidou
J. Liq. Chromatogr., 18(13), 2593-2609 (1995).
15. “Soxhlet Extraction and Acid Digestion Methods for Silver Determination
in Soils by
Flame and Electrothermal Atomic Absorption Spectrometry”
I.N. Papadoyannis, M.K. Sofoniou, V.F.
Samanidou, G.A. Zachariadis,
A.C. Zotou, H.A. Constantinidou, S.E. Tsiouris
Instrum.
Sci. Technol., 25(4), 283-296 (1997).
16. “Determination of Hypericin and Pseudohypericin
in Extracts from Hypericum Perforatum
L. and Pharmaceutical Preparations by Liquid Chromatography- Fluorescence
Detection”
A. Zotou*, Z. Loukou
Chromatographia, 54 (3/4), 218-224 (2001).
17. “Sensitive LC Determination of Ciprofloxacin in Pharmaceutical
Preparations and Biological Fluids with
Fluorescence Detection”
Anastasia Zotou*,
Niki Miltiadou
J. Pharm. Biomed. Anal., 28, 559-568 (2002).
18. “Ion-Pair High Performance Liquid Chromatographic Study of the
Hydrolysis Behaviour
of Reactive Fluorotriazinic Dyes”
A. Zotou,
Dyes Pigm., 53, 267-275 (2002).
19. “Development of a Solid Phase Extraction Protocol for the Simultaneous
Determination of Anthracene and its Oxidation Products in Surface Waters by Reversed-Phase HPLC”
I.N. Papadoyannis, A. Zotou and V.F. Samanidou
J. Liq. Chromatogr. Relat.
Technol., 25(17),
2635-2653 (2002).
20. “Determination of Biogenic Amines in Wines and Beers by High Performance
Liquid Chromatography with Pre-column Dansylation and
Ultraviolet Detection”
Á. Zotou*, Z. Loukou, E. Soufleros, I. Stratis
Chromatographia, 57(7/8), 429-439 (2003).
21. “Determination of Biogenic
Amines as Dansyl Derivatives in Alcoholic Beverages
by High-Performance Liquid Chromatography with Fluorimetric
Detection and Characterization of the Dansylated Amines by Liquid
Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry”
Zacharenia Loukou, Anastasia Zotou*
J. Chromatogr. A, 996, 103-113 (2003).
22. “A Comparative Survey of the Simultaneous Ultraviolet and Fluorescence
Detection in the RP-HPLC Determination of Dansylated Biogenic Amines in
Alcoholic Beverages”
Z. Loukou, A. Zotou*
Chromatographia, 58(9/10), 579-585
(2003).
23. “Method Development for the Determination of Seven Organic Acids in
Wines By Reversed-Phase High
Performance Liquid Chromatography”
Á. Zotou*, Z. Loukou, O. Karava
Chromatographia, 60(1/2), 39-44 (2004).
24. “Determination of 1,2-dibromoethane, 1,4-dichlorobenzene and naphthalene
Residues in Honey by Gas-Chromatography-Mass Spectrometry using Purge and Trap
Thermal Desorption Extraction”
Chrisoula Tananaki,
Anastasia Zotou* Andreas Thrasyvoulou
J. Chromatogr. A, 1083, 146-152 (2005).
25. “Contamination of honey by chemicals
applied to protect honeybee combs from wax-moth (Galleria mellonela
L.)”
Chrisoula Tananaki, Andreas Thrasyvoulou, Emmanouel Karazaphiris, Anastasia
Zotou
Food Addit. Contam., 23(2), 159-163 (2006).
26. “Selective determination of
sulfonamide residues in honey by SPE-RP-LC with UV detection”
A. Zotou*, C. Vasiliadou
Chromatographia,
64(5/6), 307-311 (2006).
27. “Determination of biogenic amines in Greek wines by HPLC and ultraviolet
detection after dansylation and examination of
factors affecting their presence and concentration”
E.H. Soufleros, E. Bouloumpasi, A. Zotou, Z. Loukou
Food Chem., 101,
704-716 (2007).
28. “The role of ascorbic acid and exercise in chronic ischemia of skeletal
muscle in rats”
T. Loizidis,
A. Sioga, L. Economou, A. Frosinis, A. Kyparos, A. Zotou, M. Albani
J.
Appl. Physiol., 102, 321-330 (2007).
29. “Optimization and
validation of a dissolution test for selegiline hydrochloride tablets by a novel rapid HPLC assay using a
monolithic stationary phase”
Paraskevas D. Tzanavaras,
Demetrius G. Themelis, Anastasia Zotou,
John Stratis,
BoKarlberg
J.
Pharm. Biom. Anal., 46, 670-675 (2008).
30. “Development and
validation of an SPE-LC method for the simultaneous determination of trans-resveratrol and selected
flavonoids in wine”
Anastasia
Zotou*, Eleni Frangi
Chromatographia, 67,
789-793 (2008).
31. “Separation and
determination of nimesulide related substances for
quality control purposes by micellar electrokinetic chromatography”
Constantinos K. Zacharis, Paraskevas D. Tzanavaras, Maria Notou, Anastasia Zotou,
Demetrius G. Themelis
J. Pharm. Biom. Anal., 49, 201-206 (2009).
32. “Determination of 1,4-dichlorobenzene
in royal jelly”
Ch.
Tananaki, G. Goras, A. Thrasyvoulou, A. Zotou
J. Apicult. Res., 48(1), 79-80 (2009)
33. “A Fluorescence-LC method
for the determination of sulfonamides in biological fluids with pre-column
derivatization”
A. Zotou*,
C. Vasiliadou
Chromatographia, 70,
389-397 (2009).
34. “LC of sulfonamide residues
in poultry muscle and eggs extracts using
fluorescence pre-column derivatization
and monolithic silica column"”
A. Zotou*, C. Vasiliadou
J.
Sep. Sci., 33, 11–22 (2010).
35. “LC-determination of five paraben
preservatives in saliva and toothpaste samples using UV detection and a short
monolithic column”
Anastasia Zotou*, Ioanna Sakla and Paraskevas D. Tzanavaras
J.
Pharm. Biomed.
Anal., 53,
785–789 (2010).
36. “Ethyl propiolate
as a post-column derivatization reagent for thiols: development of a green liquid
chromatographic method for the determination of glutathione in vegetables”
Constantinos K. Zacharis, Paraskevas D. Tzanavaras and Anastasia Zotou
Anal. Chim. Acta, 690, 122–128 (2011).
37. “Study of the
naphthalene-2,3-dicarboxaldehyde pre-column derivatization of biogenic mono-
and diamines in mixture and fluorescence-HPLC determination”
Anastasia Zotou*, Maria Notou
Anal. Bioanal.
Chem., 403,
1039-1048 (2012).
38. “An overview of recent
advances in HPLC instrumentation”
(invited review article)
Anastasia
Zotou*
Cent. Eur. J. Chem., 10(3), 554-569 (2012).
39. “Isocratic liquid chromatographic determination of three paraben
preservatives in hygiene wipes using a reversed phase core-shell narrow-bore
column”
Paraskevas D. Tzanavaras,
Theano D. Karakosta ,
Pantelis G. Rigas, Demetrius G.
Themelis, Anastasia Zotou
Cent. Eur. J. Chem., 10 (5), 1459-1463 (2012).
40. “Enhancing fluorescence-LC
analysis of biogenic amines in fish tissues by pre- column derivatization with naphthalene-2,3-dicarboxaldehyde”
Anastasia Zotou*, Maria Notou
Food Anal. Methods, 6(1), 89-99
(2013).
41. “High-throughput
determination of quinine in beverages and soft drinks based on zone-fluidics
coupled to monolithic liquid chromatography”
Paraskevas D. Tzanavaras, Constantinos K. Zacharis, Theano D. Karakosta,
Anastasia Zotou and
Demetrius G. Themelis
Anal. Letters, 46, 1718–1731 (2013).
42. “Determination
of glutathione in baker’s yeast by capillary electrophoresis using methyl propiolate as derivatizing reagent”
E.-C. Tsardaka, C. K. Zacharis, P.D. Tzanavaras, A. Zotou*
J.
Chromatogr. A, 1300,
204– 208 (2013).
43. “Assay
of biogenic monoamines in milk by fluorescence-LC following derivatization with
naphthalene-2,3-dicarboxaldehyde” (invited article)
Maria Notou,
Anastasia Zotou*
Curr. Anal. Chem., 10(3), 326-337 (2014).
44. “Automated derivatization and fluorimetric
determination of biogenic amines in
milk by zone fluidics coupled to liquid
chromatography”
Maria Notou, Anastasia Zotou*,
Paraskevas Tzanavaras, Demetrius Themelis
J. Chromatogr.
A, 1356, 272-276 (2014).
45. “Use
of a Core-shell Column for the Development of a Green LC Method for
Thiol Determination in Fresh Fruits Following
Derivatization with Methyl
Propiolate”
Alexandra
Samara, Anastasia Zotou*, Paraskevas Tzanavaras
Food Anal.
Methods, 9(3), 680-690 (2016).
46. “Determination of Tyrian purple by High Performance
Liquid Chromatography
with diode array detection”
Athina Vasileiadou, Ioannis Karapanagiotis, Anastasia
Zotou*
J. Chromatogr. A, 1448, 67-72 (2016).
47. “Use
of a monolithic column for the development and validation of a HPLC
method for the determination of famotidine,
cimetidine and nizatidine in
biological fluids”
Kontou Marina, Zotou Anastasia*
J. Applied Bioanal.,
3(4), 98-109 (2017).
48. “Profiling of hydrophilic constituents of royal jelly
by HILIC-MS/MS”
Athanasia Pina, Olga Begou,
Dimitris Kanelis, Helen Gika*,
Stavros Kalogiannis ,
Chrysa Tananaki, Georgios
Theodoridis, Anastasia Zotou **
J. Chromatogr. A,
1531, 53–63 (2018).
49. “Wine
and grape marc spirits metabolomics. A review”
Dimitra
Diamantidou, Anastasia Zotou,
Georgios Theodoridis*
Metabolomics : Official
journal of the Metabolomic Society
14(12),
pp. 159 (2018).
50. “UV-induced degradation of
wool and silk dyed with shellfish purple”
Ioannis Karapanagiotis*, Anastasia Zotou*,
Athina Vasileiadou
Dyes and Pigments,
168, 317–326 (2019).
51. “Development and validation of an ultra high performance liquid
chromatography-tandem mass spectrometry
method for the determination of
phthalate
esters in Greek grape marc spirits”
Dimitra Diamantidou, Olga Begou, Georgios Theodoridis, Helen Gika,
Emmanouil Tsochatzis,
Stavros Kalogiannis, Natalia Kataiftsi, Evangelos Soufleros, Anastasia Zotou*
J.
Chromatogr. A, 1603, 165–178 (2019).
52. “A targeted approach for
studying the effect of sugar bee feeding on the
metabolic
profile of Royal Jelly”.
Christina Virgiliou,
Dimitrios Kanelis, Athanasia Pina, Helen Gika,
Chrysoula
Tananaki,
Anastasia Zotou, Georgios Theodoridis
J. Chromatogr. A, 1616,
460783 (2020).
53. “Study of the oxidative forced degradation
of Glutathione in its nutraceutical
formulations using Zone
Fluidics and Green Liquid Chromatography”
Apostolia Tsiasioti , Constantinos K. Zacharis , Anastasia-Stella Zotou and Paraskevas D. 5 Tzanavaras
Separations, 7(1),
16 (2020). https://doi.org/10.3390/separations7010016
iii) Publications in refereed
journals without impact factor
1. “Extraction and determination of volatile compounds in honey by Purge
and Trap – Gas Chromatography and Flame Ionization Detection”
Chrisoula Tananaki, Anastasia Zotou, Andreas Thrasyvoulou
Chimica Chronica,
General Edition,4, 24-27 (2004).
1. "Qualitative Chemical
Analysis". Course Book in greek,
p.p. 382, Pegasus 2000, Thessaloniki, 1st edition 1999, 2nd
edition 2003.
2. “Bioanalytical Chemistry”.
Two chapters in
Course e-book, p.p. 136-182, ISBN: 978-960-603-052-9,
Copyright © ÓÅÁÂ, 2015, www.kallipos.gr
3. “Analytical Chemistry”. Course book in greek, p.p. 816, 1st
edition , Ziti, Thessaloniki, 2017
v) Chapters in Books and Encyclopedias
1. “Fluorescence Detection in HPLC”
Ioannis N. Papadoyannis,
Anastasia Zotou
Encyclopedia of Chromatography, Marcel Dekker, New York 2001,
pp.
338-342.
2. “Fronting of Chromatographic Peaks, Causes”
Ioannis N. Papadoyannis, Anastasia Zotou
Encyclopedia of Chromatography, Marcel Dekker, New York 2001,
pp. 362- 363.
3. “Ion-Pairing Techniques”
Ioannis N. Papadoyannis, Anastasia Zotou
Encyclopedia of Chromatography, Marcel Dekker, New York 2001,
pp. 461-
464.
4. “Methods for Determining
Biogenic Amines in Beer”
Anastasia Zotou, Zacharenia Loukou
“Beer in Health and Disease
Prevention”, Edited by Victor R. Preedy
ISBN:
978-0-12-373891-2, Elsevier Inc. 2009, Academic Press, pp.
1031-1041.
Conferences
Participation
in more than 20 international conferences, 12 national conferences, 2
international workshops and 3 seminars. One invited lecture.
Reviewer
Analytica Chimica Acta (Elsevier)
Bioanalysis (Future Science Group)
Central
European Journal of Chemistry(Versita,
co-publisher with SpringerLink )
Chromatographia (SpringerLink)
Current
Analytical Chemistry (Bentham Science
Publishers)
Journal of Agricultural and Food
Chemistry (American Chemical Society)
Journal
of Chromatographic Science (
Journal of Chromatography A (Elsevier)
Journal of Chromatography B (Elsevier):
Journal
of Food Science (Wiley)
Journal of Separation Science (Wiley Interscience):
Journal
of the Serbian Chemical Society
Mikrochimica Acta (SpingerLink)
Talanta (Elsevier)
Food Analytical Methods (SpingerLink)