Zotou Anastasia



Laboratory of Analytical Chemistry
Department of Chemistry

Aristotle University of Thessaloniki

54124 Thessaloniki, Greece

Tel.: +30 2310 997746




Postdoctoral studies: School of Pharmacy and Pharmacology, Laboratory of Pharmaceutical Analysis, University of Bath, England (12 months, 1991-92).

Ph.D.: Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki (1989).

Supplementary degree in Applied Environmental Chemistry: University of Umeå, Sweden (1980).

Degree: Philosophie Candidatexamen (equivalent to B.Sc.), University of Umeå, Sweden (1979), Major: Chemistry.


Academic status


Full Professor, February 2014-present

Aristotle University of Thessaloniki,

Department of Chemistry


Associate Professor, 2004-2014

Aristotle University of Thessaloniki,

Department of Chemistry


Assistant Professor, 1995-2004

Aristotle University of Thessaloniki,

Department of Chemistry


Lecturer, 1990-1995

Aristotle University of Thessaloniki,

Department of Chemistry


Professional Association Memberships

Member of Greek Chemists’ Association.

Member of British Chromatographic Society (1992-1995).

Member of Greek Toxicology Society.




Undergraduate level:


Basic Principles of Analytical Chemistry, Department of Chemistry, AUTh

• Instrumental Chemical Analysis I, Department of Chemistry, AUTh

• Special Methods of Analysis, Department of Chemistry, AUTh

• Bioanalytical Chemistry, Department of Chemistry, AUTh

• Analytical Chemistry, Department of Geology, AUTh

• Analytical Chemistry, Department of Agriculture, AUTh


Postgraduate level: "Quality Control-Chemical Analysis-Environmental Analysis " Department of Chemistry, AUTh


• Separation Techniques in Chemical Analysis

• Bioanalytical Methods

• Research and Laboratory Methodology in Bioanalysis

• Standard Separation Techniques in Chemical Analysis

• Research and Laboratory Methodology in Quality Control




Supervision of 4 Ph.D. students, 13 postgraduate students and 18 undergraduate students.


Current Research interests


Separation methods of analysis:

i) Reversed-Phase High Pressure and/or Ultra-High Pressure Liquid Chromatography with optical detectors and LC-MS/MS or HILIC-MS/MS.

ii) Capillary Electrophoresis with optical detection.

iii) Gas Chromatography-Mass Spectrometry. Purge-and-Trap extraction of volatile compounds.


Coupling of automated flow methods of analysis with separation techniques: Automated sample preparation by Sequential Injection Analysis coupled to Liquid Chromatography (SIA-HPLC).


Development and validation of novel analytical methods for the determination of various analytes such as amines, amino acids, organic acids, flavonoids, thiols, vitamins, drugs, e.t.c in various samples (foods and beverages, biological fluids, pharmaceuticals, e.t.c) using the above techniques.


Development and optimization of sample preparation methods: Solid Phase Extraction (SPE), Matrix Solis Phase Dispersion (MSPD), Liquid-Liquid Microextraction etc.




i) Ph.D. Dissertation


“Study of the catalytic determination of trace amounts of iron(III) and vanadium(V)”, Thessaloniki, Ed. Aristotle University of Thessaloniki, 1989.


ii) Publications in refereed journals with impact factor and in books


1. Heavy Metals in Marine Organisms of the North Aegean Sea, Greece

       G. Vasilikiotis, K. Fytianos, A. Zotou

       Chemosphere, 12(1), 75-81 (1983).

2. “Indicator Reaction for the Kinetic-Spectrophotometric Determination of Nanogram Amounts of Iron”

      Anastasia Ch. Zotou and Constantine G. Papadopoulos

      Analyst,  112, 787-789 (1987).

3. “Kinetic-Spectrophotometric Determination of Trace Amounts of Vanadium(V)”

       Anastasia Ch. Zotou and Constantine G. Papadopoulos

       Analyst, 115, 323-327 (1990).                  

4. “Rapid Assay for the Determination of Tolfenamic Acid in Pharmaceutical  Preparations and Biological Fluids by High-Performance Liquid Chromatography”

      É. Papadoyannis, M. Georgarakis, V. Samanidou and A. Zotou

     J. Liq. Chromatogr., 14(15),  2951-2967 (1991).

5. “Kinetic-Spectrophotometric Determination of Molybdenum(VI) and Tungsten(VI)

  in Mixtures

      Constantine G. Papadopoulos and Anastasia C. Zotou*

      Mikrochim. Acta, 106,  203-210 (1992).

6. “Simultaneous Reversed-Phase Gradient-HPLC Analysis of Anthranilic Acid Derivatives in Anti-Inflammatory Drugs and Samples of Biological Interest”

      I.N. Papadoyannis, A.C. Zotou and V.F. Samanidou

     J. Liq. Chromatogr.,  15(11),  1923-1945 (1992).

7. “Determination of Anatoxin-a and Homoanatoxin in Blue-Green Algal  Extracts by High-Performance Liquid Chromatography and Gas Chromatography-Mass Spectrometry”

       Anastasia Zotou, Terry M. Jefferies, Paul A. Brough and Timothy Gallagher

       Analyst,  118, 753-758 (1993).

8. “Comparative Study of Different Solid-Phase Extraction Cartridges in the Simultaneous RP-HPLC Analysis of Morphine and Codeine in Biological Fluids”

      I. Papadoyannis, A. Zotou, V. Samanidou, G. Theodoridis and F. Zougrou

     J.  Liq. Chromatogr.,  16(14),  3017-3040 (1993).

9. “Comparative Study of Solid-Phase Extraction Cartridges in the Simultaneous Reversed-Phase HPLC Analysis of Bamifylline and its Major Metabolite AC-119 in Biological Fluids”

     I. Papadoyannis, V. Samanidou, A. Zotou and G. Tsioni

     J. Liq. Chromatogr.,  16(17),  3827-3845 (1993).

10. “Simultaneous Determination of Bamifylline and its Major Metabolite AC-119  

  by RP- HPLC”

       I. Papadoyannis, V. Samanidou and A. Zotou

       J. Liq. Chromatogr., 16(17), 3847-3861 (1993).

11. “Determination of Anatoxin-a, Homoanatoxin and Propylanatoxin in Cyanobacterial Extracts by HPLC, GC-Mass Spectrometry and Capillary Electrophoresis”

    Terry M. Jefferies, Gavin Brammer, Anastasia Zotou, Paul A. Brough and Timothy


    In "Detection Methods for Cyanobacterial Toxins", The Royal Society of Chemistry,

1994, pp. 34-39.

12. “Solid-Phase Extraction and RP-HPLC Analysis of Atropine Sulphate and  Scopolamine-N-Butylbromide in Pharmaceutical Preparations and Biological Fluids”

       I. Papadoyannis, A. Zotou, V. Samanidou and M. Georgarakis

    Instrum. Sci. Technol., 22(1),  83-103 (1994).

13. “Highly Selective Simultaneous Determination of eight Inorganic Anions in Drinking Water by Single Column High Pressure Anion Chromatography”

      I. Papadoyannis, V. Samanidou and A. Zotou

      J.  Liq. Chromatogr.,  18(7), 1383-1403  (1995).

14. “Solid-Phase Extraction Study and RP-HPLC Analysis of Lamotrigine in Human Biological Fluids and in Antiepileptic Tablet Formulations”

      I.N. Papadoyannis, A.C. Zotou and V.F. Samanidou

       J. Liq. Chromatogr.,  18(13),  2593-2609 (1995).

15. “Soxhlet Extraction and Acid Digestion Methods for Silver Determination in Soils by Flame and Electrothermal Atomic Absorption Spectrometry”

      I.N. Papadoyannis, M.K. Sofoniou, V.F. Samanidou, G.A. Zachariadis,

      A.C. Zotou, H.A. Constantinidou, S.E. Tsiouris

   Instrum. Sci. Technol., 25(4), 283-296 (1997).

16. “Determination of Hypericin and Pseudohypericin in Extracts from Hypericum Perforatum L. and Pharmaceutical Preparations by Liquid Chromatography- Fluorescence Detection”

      A. Zotou*, Z. Loukou

      Chromatographia,  54 (3/4), 218-224 (2001).

17. “Sensitive LC Determination of Ciprofloxacin in Pharmaceutical Preparations and  Biological Fluids with Fluorescence Detection”

     Anastasia Zotou*, Niki Miltiadou

     J. Pharm. Biomed. Anal.,  28, 559-568 (2002).

18. “Ion-Pair High Performance Liquid Chromatographic Study of the Hydrolysis  Behaviour of Reactive Fluorotriazinic Dyes”

     A. Zotou, I. Eleftheriadis, M. Heli, S. Pegiadou

     Dyes Pigm.,  53, 267-275  (2002).

19. “Development of a Solid Phase Extraction Protocol for the Simultaneous Determination of Anthracene and its Oxidation Products in Surface Waters by Reversed-Phase HPLC”

     I.N. Papadoyannis, A. Zotou and V.F. Samanidou

     J. Liq. Chromatogr. Relat. Technol.,  25(17), 2635-2653 (2002).

20. “Determination of Biogenic Amines in Wines and Beers by High Performance Liquid Chromatography with Pre-column Dansylation and Ultraviolet Detection”

     Á. Zotou*, Z. Loukou, E. Soufleros, I. Stratis

     Chromatographia,  57(7/8), 429-439 (2003).

21. “Determination of Biogenic Amines as Dansyl Derivatives in Alcoholic Beverages by High-Performance Liquid Chromatography with Fluorimetric Detection and Characterization of the Dansylated Amines by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry”

     Zacharenia Loukou, Anastasia Zotou*

     J. Chromatogr. A,  996, 103-113  (2003).

22. “A Comparative Survey of the Simultaneous Ultraviolet and Fluorescence Detection in the RP-HPLC Determination of Dansylated Biogenic Amines in Alcoholic Beverages

     Z. Loukou, A. Zotou*

     Chromatographia, 58(9/10),  579-585 (2003).

23. “Method Development for the Determination of Seven Organic Acids in Wines  By Reversed-Phase High Performance Liquid Chromatography”

     Á. Zotou*, Z. Loukou, O. Karava

     Chromatographia, 60(1/2), 39-44 (2004).

24. “Determination of 1,2-dibromoethane, 1,4-dichlorobenzene and naphthalene Residues in Honey by Gas-Chromatography-Mass Spectrometry using Purge and Trap Thermal Desorption Extraction”

     Chrisoula Tananaki, Anastasia Zotou* Andreas Thrasyvoulou

     J. Chromatogr. A, 1083, 146-152 (2005).

25. Contamination of honey by chemicals applied to protect honeybee combs from wax-moth (Galleria mellonela L.)”

     Chrisoula Tananaki, Andreas Thrasyvoulou, Emmanouel Karazaphiris, Anastasia Zotou

     Food Addit. Contam., 23(2), 159-163 (2006).

26. “Selective determination of sulfonamide residues in honey by SPE-RP-LC with UV detection”

     A. Zotou*, C. Vasiliadou

     Chromatographia, 64(5/6), 307-311 (2006).

27. “Determination of biogenic amines in Greek wines by HPLC and ultraviolet detection after dansylation and examination of factors affecting their presence and concentration”

E.H. Soufleros, E. Bouloumpasi, A. Zotou, Z. Loukou

Food Chem., 101, 704-716 (2007).

28. “The role of ascorbic acid and exercise in chronic ischemia of skeletal muscle in rats”

T. Loizidis, A. Sioga, L. Economou, A. Frosinis, A. Kyparos, A. Zotou, M. Albani

 J. Appl. Physiol., 102, 321-330 (2007).

29. “Optimization and validation of a dissolution test for selegiline hydrochloride  tablets by a novel rapid HPLC assay using a monolithic stationary phase”

     Paraskevas D. Tzanavaras, Demetrius G. Themelis, Anastasia Zotou, John Stratis,


      J. Pharm. Biom. Anal., 46, 670-675 (2008).

30. “Development and validation of an SPE-LC method for the simultaneous determination of trans-resveratrol and selected flavonoids in wine”

   Anastasia Zotou*, Eleni Frangi

     Chromatographia, 67, 789-793 (2008).

31.    “Separation and determination of nimesulide related substances for quality control purposes by micellar electrokinetic chromatography”

     Constantinos K. Zacharis, Paraskevas D. Tzanavaras, Maria Notou, Anastasia Zotou,

     Demetrius G. Themelis

     J. Pharm. Biom. Anal., 49, 201-206 (2009).

32. “Determination of 1,4-dichlorobenzene in royal jelly”

Ch. Tananaki, G. Goras, A. Thrasyvoulou, A. Zotou

     J. Apicult. Res., 48(1), 79-80 (2009)

33. “A Fluorescence-LC method for the determination of sulfonamides in biological fluids with pre-column derivatization”

     A. Zotou*, C. Vasiliadou

        Chromatographia,  70, 389-397 (2009).

34. LC of sulfonamide residues in poultry muscle and eggs extracts using
 fluorescence pre-column derivatization and monolithic silica column"”

      A. Zotou*, C. Vasiliadou

      J. Sep. Sci., 33, 11–22 (2010).

35. LC-determination of five paraben preservatives in saliva and toothpaste samples using UV detection and a short monolithic column

     Anastasia Zotou*, Ioanna Sakla and Paraskevas D. Tzanavaras                               

   J. Pharm. Biomed. Anal., 53, 785–789 (2010).

36. “Ethyl propiolate as a post-column derivatization reagent for thiols: development of a green liquid chromatographic method for the determination of glutathione in  vegetables

       Constantinos K. Zacharis, Paraskevas D. Tzanavaras and Anastasia Zotou

       Anal. Chim. Acta,  690, 122–128 (2011).

37.    “Study of the naphthalene-2,3-dicarboxaldehyde pre-column derivatization of biogenic mono- and diamines in mixture and fluorescence-HPLC determination”

Anastasia Zotou*, Maria Notou

Anal. Bioanal. Chem., 403, 1039-1048 (2012).

38. “An overview of recent advances in HPLC  instrumentation” (invited review  article)

Anastasia Zotou*

 Cent. Eur. J. Chem., 10(3), 554-569 (2012).

39. “Isocratic liquid chromatographic determination of three paraben preservatives in hygiene wipes using a reversed phase core-shell narrow-bore column”

Paraskevas D. Tzanavaras, Theano D. Karakosta ,

Pantelis G. Rigas, Demetrius G. Themelis,  Anastasia Zotou

Cent. Eur. J. Chem., 10 (5), 1459-1463 (2012).

40. “Enhancing fluorescence-LC analysis of biogenic amines in fish tissues by pre- column derivatization with naphthalene-2,3-dicarboxaldehyde”

Anastasia Zotou*, Maria Notou

Food Anal. Methods, 6(1), 89-99 (2013).      

41. “High-throughput determination of quinine in beverages and soft drinks based on zone-fluidics coupled to monolithic liquid chromatography”

     Paraskevas D. Tzanavaras, Constantinos K. Zacharis, Theano D. Karakosta,

Anastasia Zotou and Demetrius G. Themelis

     Anal. Letters, 46, 1718–1731 (2013).

42. “Determination of glutathione in baker’s yeast by capillary electrophoresis using methyl propiolate as derivatizing reagent”

E.-C. Tsardaka, C. K. Zacharis, P.D. Tzanavaras, A. Zotou*

J. Chromatogr. A,  1300, 204– 208 (2013).

43. “Assay of biogenic monoamines in milk by fluorescence-LC following derivatization with naphthalene-2,3-dicarboxaldehyde” (invited article)

     Maria Notou, Anastasia Zotou*

     Curr. Anal. Chem., 10(3), 326-337 (2014).

44. “Automated derivatization and fluorimetric determination of biogenic amines in

  milk by zone fluidics coupled to liquid chromatography”

      Maria Notou, Anastasia Zotou*,  Paraskevas Tzanavaras, Demetrius Themelis

        J. Chromatogr. A, 1356, 272-276 (2014).

45. “Use of a Core-shell Column for the Development of a Green LC Method for

  Thiol Determination in Fresh Fruits Following Derivatization with Methyl


   Alexandra Samara, Anastasia Zotou*, Paraskevas Tzanavaras

    Food Anal. Methods, 9(3), 680-690 (2016).

46.  “Determination of Tyrian purple by High Performance Liquid Chromatography

with diode array detection”

Athina Vasileiadou, Ioannis Karapanagiotis, Anastasia Zotou*

J. Chromatogr. A, 1448, 67-72 (2016).

47.    “Use of a monolithic column for the development and validation of a HPLC

   method for the determination of famotidine, cimetidine and nizatidine in

   biological fluids”

 Kontou Marina, Zotou Anastasia*

  J. Applied Bioanal., 3(4), 98-109 (2017).

48.    “Profiling of hydrophilic constituents of royal jelly by HILIC-MS/MS”

          Athanasia Pina, Olga Begou, Dimitris Kanelis, Helen Gika*, Stavros Kalogiannis ,

      Chrysa Tananaki, Georgios Theodoridis, Anastasia Zotou **

          J. Chromatogr. A, 1531, 53–63 (2018).

49.    Wine and grape marc spirits metabolomics. A review”

Dimitra Diamantidou, Anastasia Zotou, Georgios Theodoridis*

Metabolomics : Official journal of the Metabolomic Society

14(12), pp. 159 (2018).

50.    “UV-induced degradation of wool and silk dyed with shellfish purple”

Ioannis Karapanagiotis*, Anastasia Zotou*, Athina Vasileiadou

Dyes and Pigments, 168, 317–326 (2019).

51.   “Development and validation of an ultra high performance liquid

   chromatography-tandem mass spectrometry method for the determination of

phthalate esters in Greek grape marc spirits”

Dimitra Diamantidou, Olga Begou, Georgios Theodoridis, Helen Gika, Emmanouil Tsochatzis, Stavros Kalogiannis, Natalia Kataiftsi, Evangelos Soufleros, Anastasia Zotou*

J. Chromatogr. A, 1603, 165–178 (2019).

52.    “A targeted approach for studying the effect of sugar bee feeding on the

  metabolic profile of Royal Jelly”.

  Christina Virgiliou,  Dimitrios Kanelis, Athanasia Pina, Helen Gika, Chrysoula  

   Tananaki, Anastasia Zotou, Georgios Theodoridis

  J. Chromatogr. A, 1616, 460783 (2020).

53.    “Study of the oxidative forced degradation of  Glutathione in its nutraceutical

   formulations using  Zone Fluidics and Green Liquid Chromatography”  

Apostolia Tsiasioti , Constantinos K. Zacharis , Anastasia-Stella Zotou  and Paraskevas D. 5 Tzanavaras

Separations, 7(1), 16 (2020).


iii) Publications in refereed journals without impact factor


1. “Extraction and determination of volatile compounds in honey by Purge and Trap – Gas Chromatography and Flame Ionization Detection”

      Chrisoula Tananaki, Anastasia Zotou, Andreas Thrasyvoulou  

      Chimica Chronica, General Edition,4,  24-27 (2004).

iv) Textbooks

1.  "Qualitative Chemical Analysis". Course Book in greek, p.p. 382, Pegasus 2000, Thessaloniki, 1st edition 1999, 2nd edition 2003.

2.  “Bioanalytical Chemistry”. Two chapters in Course e-book, p.p. 136-182, ISBN: 978-960-603-052-9, Copyright © ÓÅÁÂ, 2015,

3.  “Analytical Chemistry”. Course book in greek, p.p. 816, 1st edition , Ziti, Thessaloniki, 2017


v) Chapters in Books and Encyclopedias


1.  “Fluorescence Detection in HPLC”

        Ioannis N. Papadoyannis, Anastasia Zotou

       Encyclopedia of Chromatography,  Marcel Dekker, New York 2001,

       pp. 338-342.

2.  “Fronting of Chromatographic Peaks, Causes”

 Ioannis N. Papadoyannis, Anastasia Zotou

      Encyclopedia of Chromatography,  Marcel Dekker, New York 2001,

      pp. 362- 363.

3.  “Ion-Pairing Techniques”

 Ioannis N. Papadoyannis, Anastasia Zotou

       Encyclopedia of Chromatography,  Marcel Dekker, New York 2001,

        pp. 461- 464.

4.  “Methods for Determining Biogenic Amines in Beer”

           Anastasia Zotou, Zacharenia Loukou

       Beer in Health and Disease Prevention”, Edited by Victor R. Preedy

       ISBN: 978-0-12-373891-2, Elsevier Inc. 2009, Academic Press, pp.




Participation in more than 20 international conferences, 12 national conferences, 2 international workshops and 3 seminars. One invited lecture.




Analytica  Chimica  Acta  (Elsevier)

Bioanalysis (Future Science Group)

Central European Journal of Chemistry(Versita, co-publisher with SpringerLink )

Chromatographia (SpringerLink)

Current Analytical Chemistry (Bentham Science Publishers)

Journal of Agricultural and Food Chemistry (American Chemical Society)

Journal of Chromatographic Science (Preston Publications)

Journal of  Chromatography A (Elsevier)

Journal of Chromatography B (Elsevier):

Journal of Food Science (Wiley)

Journal of Separation Science (Wiley Interscience):

Journal of the Serbian Chemical Society

Mikrochimica Acta (SpingerLink)

Talanta (Elsevier)

Food Analytical Methods (SpingerLink)